caa a22 4500 477067271 CHVBK 20180405111416.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02458388 doi (NATIONALLICENCE)springer-10.1007/BF02458388 A novel intact circular dsDNA supercoil [Elektronische Daten] [Richard Wu, Tai Te Wu] A novel intact circular dsDNA supercoil is proposed as an alternative to the conventional DNA supercoil, so that the two complementary strands of ssDNA circles are separable without any covalent bond breakage. This new structure can be visualized by using two tubings: one black and one clear. Twist the black tubing a number of times and connect its two ends. Do the same for the clear tubing. Then wrap the two tubings together. This forms the separable or novel supercoil. On the other hand, the conventional supercoil can be modeled by twisting the black and clear tubings together and then connect their respective ends, so that the two tubings are not separable unless one of them is cut. Experimentally, in the absence of any enzyme, many intact plasmid dsDNA circles give two bands on agarose gel electrophoresis under a certain given condition, while the same plasmid molecules after cutting once by a restriction enzyme give only one band under the same, condition. In the case of intact pUC19 plasmids, these two bands can then be, recovered and sequenced separately, using two primers in opposite directions. Each band gives mostly one sequence which is complementary to that of the other band. The combination of the above theoretical model and experimental results strongly suggests that there is an alternative structure of DNA which does not have the usual difficulty of unwinding, rewinding and requiring numerous covalent bond breakages and ligations during semiconservative replication. Society for Mathematical Biology, 1996 Wu Richard Departments of Biochemistry, Molecular Biology and Cell Biology, and Department of Biomedical Engineering, Northwestern University, 60208, Evanston, IL, U.S.A. aut Te Wu Tai Departments of Biochemistry, Molecular Biology and Cell Biology, and Department of Biomedical Engineering, Northwestern University, 60208, Evanston, IL, U.S.A. aut Bulletin of Mathematical Biology Kluwer Academic Publishers 58/6(1996-11-01), 1171-1185 0092-8240 58:6<1171 1996 58 11538 https://doi.org/10.1007/BF02458388 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02458388 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Richard Departments of Biochemistry, Molecular Biology and Cell Biology, and Department of Biomedical Engineering, Northwestern University, 60208, Evanston, IL, U.S.A aut NATIONALLICENCE 700 1- Te Wu Tai Departments of Biochemistry, Molecular Biology and Cell Biology, and Department of Biomedical Engineering, Northwestern University, 60208, Evanston, IL, U.S.A aut NATIONALLICENCE 773 0- Bulletin of Mathematical Biology Kluwer Academic Publishers 58/6(1996-11-01), 1171-1185 0092-8240 58:6<1171 1996 58 11538 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer