caa a22 4500 477069533 CHVBK 20180405111421.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01586183 doi (NATIONALLICENCE)springer-10.1007/BF01586183 Are outbreaks and sporadic respiratory infections by Mycoplasma pneumoniae due to two distinct subtypes? [Elektronische Daten] [E. Jacobs, M. Vonski, K. Oberle, O. Opitz, K. Pietsch] Thirty-seven clinical isolates ofMycoplasma pneumoniae, cultured from patients' respiratory material between 1986 and 1994, were typed by immunological methods and by polymerase chain reaction (PCR). For immunological typing two monoclonal antibodies (mAb) were used that recognized the P1 adhesion ofMycoplasma pneumoniae strain FH but differed in their ability to inhibit the adherence ofMycoplasma pneumoniae to erythrocytes. The mAb P1.58, which was not able to inhibit adherence, showed reactions with all patients' isolates in immunoblots, whereas the adherence-inhibiting mAb P1.62 reacted with only seven patients' isolates. Due to variations within the P1adhesin genome ofMycoplasma pneumoniae group 1 (Mycoplasma pneumoniae type strain M129) and group 2 (Mycoplasma pneumoniae type strain FH), two primer sets were designed. According to the size of the PCR-amplification products, all clinical isolates that showed no mAb P1.62 reactivity belonged toMycoplasma pneumoniae group 1, whereas mAb P1.62-positive-reacting mycoplasma isolates were characterized as group 2 strains. During an outbreak ofMycoplasma pneumoniae diseases in 1992, all 19 clinical isolates showed no cross-reactivity in immunoblots with the mAb P1.62 and were typed by PCR asMycoplasma pneumoniae group 1 strains. Furthermore, 206Mycoplasma pneumoniae complement fixation test — positive patient sera (titer>1∶40) from the study period were tested for adherence-inhibiting antibodies towards both type strains. Thirty-two sera showed adherence-inhibiting antibodies towards group 1 and 22 towards group 2 mycoplasmas. In only seven sera were adherence-inhibiting antibodies directed to bothMycoplasma pneumoniae groups. The serological data of the outbreak in 1992 revealed that patients withMycoplasma pneumoniae group 1 infections developed adherence-inhibiting antibodies more frequently than did patients infected with group 2, which might have implications for the pathogenesis ofMycoplasma pneumoniae diseases and subsequent infections. Springer-Verlag, 1996 Jacobs E. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut Vonski M. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut Oberle K. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut Opitz O. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut Pietsch K. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 38-44 0934-9723 15:1<38 1996 15 10096 https://doi.org/10.1007/BF01586183 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01586183 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jacobs E. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Vonski M. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Oberle K. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Opitz O. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Pietsch K. Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 38-44 0934-9723 15:1<38 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer