caa a22 4500 477069541 CHVBK 20180405111421.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01586184 doi (NATIONALLICENCE)springer-10.1007/BF01586184 Performance of European laboratories testing serum samples for Toxoplasma gondii [Elektronische Daten] [J. Petithory, I. Reiter-Owona, F. Berthelot, M. Milgram, J. De Loye, E. Petersen] One hundred twenty-nine European laboratories participated in a collaborative, multicentre study designed to evaluate the overall reliability of different serological techniques for diagnosis ofToxoplasma gondii infection. Five freeze-dried reference sera were distributed to each laboratory, each of which analysed the sera with its routine methods. The enzyme-linked immunosorbent assay was the technique used most frequently, followed by the immunofluorescent antibody technique. Only nine laboratories performed the Sabin-Feldman dye test. In general, there was good concordance between qualitative results, but for sera with low concentrations ofToxoplasma gondii-specific IgG antibodies, some false-negative results were found. For specific IgM and IgA antibodies, the immunosorbent agglutination assay proved the most sensitive. The present study demonstrates the need for regular assessment of laboratory serodiagnosis ofToxoplasma gondii infection. Springer-Verlag, 1996 Petithory J. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut Reiter-Owona I. Institut für Parasitologie, Universität Bonn, Bonn, Germany aut Berthelot F. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut Milgram M. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut De Loye J. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut Petersen E. Laboratory of Parasitology and FAO/WHO International Centre for Research and Reference on Toxoplasmosis, Statens Seruminstitut, 2300, Copenhagen S, Denmark aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 45-49 0934-9723 15:1<45 1996 15 10096 https://doi.org/10.1007/BF01586184 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01586184 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petithory J. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut NATIONALLICENCE 700 1- Reiter-Owona I. Institut für Parasitologie, Universität Bonn, Bonn, Germany aut NATIONALLICENCE 700 1- Berthelot F. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut NATIONALLICENCE 700 1- Milgram M. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut NATIONALLICENCE 700 1- De Loye J. Centre Hospitalier, Contrôle de Qualité National en Parasitologie, 95500, Gonesse, France aut NATIONALLICENCE 700 1- Petersen E. Laboratory of Parasitology and FAO/WHO International Centre for Research and Reference on Toxoplasmosis, Statens Seruminstitut, 2300, Copenhagen S, Denmark aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 45-49 0934-9723 15:1<45 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer