caa a22 4500 477069584 CHVBK 20180405111421.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01586185 doi (NATIONALLICENCE)springer-10.1007/BF01586185 In vitro conjugative transfer of VanA vancomycin resistance between Enterococci and Listeriae of different species [Elektronische Daten] [F. Biavasco, E. Giovanetti, A. Miele, C. Vignaroli, B. Facinelli, P. Varaldo] In a study designed to gain data on the in vitro transferability of vancomycin resistance from enterococci of the VanA phenotype to listeriae of different species, three clinicalEnterococcus isolates —Enterococcus faecium LS10,Enterococcus faecalis LS4, andEnterococcus faecalis A3208, all harboring a plasmid that strongly hybridized with avanA probe — were used as donors in transfer experiments. Strains of fiveListeria species were used as recipients. FromEnterococcus faecium LS10, glycopeptide resistance was transferred toListeria monocytogenes, Listeria ivanovii, andListeria welshimeri recipients, whereas no transfer occurred toListeria seeligeri orListeria innocua strains. From the twoEnterococcus faecalis isolates, no transfer occurred to anyListeria recipient. MICs of both vancomycin and teicoplanin were ≥256 mg/l for all transconjugants tested. Furthermore, all transconjugants harbored a plasmid that strongly hybridized with thevanA probe, withvanA consistently located in anEcoRI fragment of about 4 kb. Exposure ofListeria transconjugants to vancomycin resulted in synthesis of a membrane protein similar in size (39 kDa) to a vancomycin-induced membrane protein ofEnterococcus faecium LS10. In retransfer experiments withListeria transconjugants used as donors, glycopeptide resistance was transferred to allListeria recipients tested, including strains ofListeria innocua andListeria seeligeri, which were unable to receive the resistance fromEnterococcus faecium LS10. The frequency ofvanA transfer to listerial recipients was greater in retransfer experiments than in the primary matings. These findings suggest that thevanA resistance determinant might spread to the established pathogenListeria monocytogenes, both directly from a resistant enterococcus and through strains of nonpathogenicListeria species acting as intermediate resistance vehicles. Springer-Verlag, 1996 Biavasco F. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut Giovanetti E. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut Miele A. Lepetit Research Center, Marion Merrel Dow Research Institutes, Via Lepetit 34, 21040, Gerenzano, Italy aut Vignaroli C. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut Facinelli B. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut Varaldo P. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 50-59 0934-9723 15:1<50 1996 15 10096 https://doi.org/10.1007/BF01586185 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01586185 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Biavasco F. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut NATIONALLICENCE 700 1- Giovanetti E. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut NATIONALLICENCE 700 1- Miele A. Lepetit Research Center, Marion Merrel Dow Research Institutes, Via Lepetit 34, 21040, Gerenzano, Italy aut NATIONALLICENCE 700 1- Vignaroli C. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut NATIONALLICENCE 700 1- Facinelli B. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut NATIONALLICENCE 700 1- Varaldo P. Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 50-59 0934-9723 15:1<50 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer