caa a22 4500 477069657 CHVBK 20180405111421.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01586186 doi (NATIONALLICENCE)springer-10.1007/BF01586186 Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism [Elektronische Daten] [H. Frénay, A. Bunschoten, L. Schouls, W. van Leeuwen, C. Vandenbroucke-Grauls, J. Verhoef, F. Mooi] The polymorphic X-region of the protein A gene (spa) was used for molecular typing of methicillin-resistantStaphylococcus aureus (MRSA) strains. The X-region is characterized by a variable number (between 3 and 15) of small repeats. DNA sequencing of MRSA strains revealed 25 distinct repeats. Analysis of MRSA strains grown in vitro and in vivo revealed that the X-region was sufficiently stable for epidemiologic typing of MRSA strains.Spa typing of MRSA strains was compared to phage typing and, in general, concordance was found between the two methods. However,spa typing was more sensitive, allowing differentiation of strains within a particular phage type. Results obtained withspa typing suggest that hospital outbreaks may be caused by two or more MRSA strains.Spa typing may be an important tool in unravelling the spread of MRSA strains within and between hospitals. Springer-Verlag, 1996 Frénay H. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut Bunschoten A. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut Schouls L. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut van Leeuwen W. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut Vandenbroucke-Grauls C. Eijkman-Winkler Institute of Medical Microbiology, University Hospital, Utrecht, The Netherlands aut Verhoef J. Eijkman-Winkler Institute of Medical Microbiology, University Hospital, Utrecht, The Netherlands aut Mooi F. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 60-64 0934-9723 15:1<60 1996 15 10096 https://doi.org/10.1007/BF01586186 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01586186 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frénay H. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Bunschoten A. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Schouls L. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- van Leeuwen W. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Vandenbroucke-Grauls C. Eijkman-Winkler Institute of Medical Microbiology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Verhoef J. Eijkman-Winkler Institute of Medical Microbiology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Mooi F. Molecular Microbiology Unit and Laboratory of Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, P. O. Box 1, 3720, BA Bilthoven, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/1(1996-01-01), 60-64 0934-9723 15:1<60 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer