caa a22 4500 477069797 CHVBK 20180405111422.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01691314 doi (NATIONALLICENCE)springer-10.1007/BF01691314 Modified latex agglutination test for rapid detection of Streptococcus pneumoniae and Haemophilus influenzae in cerebrospinal fluid and direct serotyping of Streptococcus pneumoniae [Elektronische Daten] [A. Singhal, M. Lalitha, T. John, K. Thomas, P. Raghupathy, S. Jacob, M. Steinhoff] A modified latex agglutination test was designed and evaluated for the rapid detection ofStreptococcus pneumoniae andHaemophilus influenzae type b capsular antigens, and for direct serotyping ofStreptococcus pneumoniae in the cerebrospinal fluid. Reagents were prepared by sensitizing latex particles with Omniserum (against 83 capsular serotypes of pneumococci) andHaemophilus influenzae type b burro antiserum. For serotyping reagents, latex particles were similarly coated with nine pneumococcal pool (A to I) antisera and 46 individual pneumococcal serogroup/serotype specific antisera. The test was performed on cerebrospinal fluid from 298 patients with suspected meningitis. Serotyping was done directly on untreated cerebrospinal fluid samples showing positive reactions with the Omniserum reagent. Pneumococcal orHaemophilus influenzae type b antigens were detected in 41 patients; in 32 of these the etiology was established by culture and in 2 by smear examination. Five of the remaining seven cases were judged clinically and by cytological examination of cerebrospinal fluid to have partially treated bacterial meningitis. In two cases the test was false positive. The overall sensitivity and specificity of the latex agglutination test for the detection ofStreptococcus pneumoniae andHaemophilus influenzae type b antigens was 100% and 96.6% respectively. The commonest pneumococcal serotypes were type 1 (30%), types 6 and 19 (10% each). The latex agglutination test is rapid and simple to perform, yielding serotype data directly by testing of cerebrospinal fluid. MMV Medizin Verlag GmbH, 1996 Singhal A. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut Lalitha M. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut John T. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut Thomas K. Medicine Unit II and Department of Clinical Epidemiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut Raghupathy P. Child Health Units I and II, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut Jacob S. Child Health Units I and II, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut Steinhoff M. Department of International Health, Johns Hopkins University, Baltimore, Maryland, USA aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/6(1996-06-01), 472-477 0934-9723 15:6<472 1996 15 10096 https://doi.org/10.1007/BF01691314 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01691314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singhal A. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- Lalitha M. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- John T. Department of Microbiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- Thomas K. Medicine Unit II and Department of Clinical Epidemiology, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- Raghupathy P. Child Health Units I and II, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- Jacob S. Child Health Units I and II, Christian Medical College and Hospital, Vellore 632 004, Tamilnadu, India aut NATIONALLICENCE 700 1- Steinhoff M. Department of International Health, Johns Hopkins University, Baltimore, Maryland, USA aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/6(1996-06-01), 472-477 0934-9723 15:6<472 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer