caa a22 4500 477069894 CHVBK 20180405111422.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01691963 doi (NATIONALLICENCE)springer-10.1007/BF01691963 Evidence of labile inhibitors in the detection of Chlamydia trachomatis in cervical specimens by polymerase chain reaction [Elektronische Daten] [A. Clad, L. Naudascher, U. Flecken, H. Freidank, E. Petersen] A total of 276 cervical swabs (241 from first visits and 35 from follow-up visits) from 241 women were tested forChlamydia trachomatis by polymerase chain reaction (PCR) and enzyme immunoassay (EIA). Sixty-one smears (53 from first visits and 8 from follow-up visits) from 53 women were stained by direct fluorescent antibody (DFA). Twenty-one (8.7%) women had positive swabs in at least two different tests. All follow-up swabs (collected between 3 days and 3 weeks after the first clinical visit) were positive in at least one test when the woman had been positive at the first visit and no antibiotic treatment had been initiated. Including swabs from follow-up visits and DFA results, the respective sensitivities and specificities of the assays were as follows: PCR, 75.9% and 100%; EIA, 69% and 98.4%. The seven swabs that were false negative by PCR (tested initially after thawing from-20°C) were mailed nonrefrigerated to the assay manufacturer, where they tested true positive. These data point to labile inhibitors of the PCR, predominantly cervical mucus. MMV Medizin Verlag GmbH, 1996 Clad A. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut Naudascher L. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut Flecken U. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut Freidank H. Institut für Medizinische Mikrobiologie, Hermann-Herderstr. 11, D-79104, Freiburg, Germany aut Petersen E. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/9(1996-09-01), 744-747 0934-9723 15:9<744 1996 15 10096 https://doi.org/10.1007/BF01691963 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01691963 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clad A. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Naudascher L. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Flecken U. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Freidank H. Institut für Medizinische Mikrobiologie, Hermann-Herderstr. 11, D-79104, Freiburg, Germany aut NATIONALLICENCE 700 1- Petersen E. Frauenklinik der Universität Freiburg, Hugstetterstr. 55, D-79106, Freiburg, Germany aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/9(1996-09-01), 744-747 0934-9723 15:9<744 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer