caa a22 4500 477070094 CHVBK 20180405111422.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01709365 doi (NATIONALLICENCE)springer-10.1007/BF01709365 Mulder J. Regional Public Health Laboratory, Van Ketwich Verschuurlaan 92, 9721, SW Groningen, The Netherlands aut Comparison of disk diffusion, the E test, and detection of mecA for determination of methicillin resistance in coagulase-negative staphylococci [Elektronische Daten] [J. Mulder] The aim of this study was to find a reliable, fast, and simple alternative to the methicillin disk method for determination of methicillin resistance in coagulase-negative staphylococci, since results of this method are often difficult to read due to growth within the zone of inhibition. The sensitivity of 319 strains of coagulase-negative Staphylococci to a 5 μg methicillin disk on Mueller-Hinton agar using an incubation period of 48 h was compared with that of 1 (1 μg and 5 μg oxacillin disks on Mueller-Hinton agar with or without 2% NaCl, using an incubation period of 24 h. The detection ofmecA (MecAgen) by the polymerase chain reaction was used as a standard. Minimum inhibitory concentrations were determined by means of the E test. Of the 225mecA-positive strains, 190, 215, and 193 were resistant to 5 μg methicillin, 1 μg oxacillin and 5 μg oxacillin disks on Mueller-Hinton agar, respectively, and 216, 218, and 223 were resistant on Mueller-Hinton agar with 2% NaCl. Of the 94mecA-negative strains, 89, 93, and 94 were susceptible to 5 μg methicillin, 1 μg oxacillin, and 5 μg oxacillin disks on Mueller-Hinton agar, respectively, and 92, 93, and 94 were susceptible on Mueller-Hinton agar with 2% NaCl. Using breakpoints of 2 μg/ml for oxacillin resistance and 8 μg/ml for methicillin resistance, the E test yielded sensitivities of 99.6 and 99.1% and specificities of 97.9 and 98.9% after 48 h of incubation. The 5 μg oxacillin disk was faster and easier to read than the methicillin disk and correlated better with detection ofmecA than the methicillin disk or the 1 μg oxacillin disk. MMV Medizin Verlag GmbH, 1996 European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 567-573 0934-9723 15:7<567 1996 15 10096 https://doi.org/10.1007/BF01709365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01709365 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Mulder J. Regional Public Health Laboratory, Van Ketwich Verschuurlaan 92, 9721, SW Groningen, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 567-573 0934-9723 15:7<567 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer