caa a22 4500 477070124 CHVBK 20180405111422.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01709372 doi (NATIONALLICENCE)springer-10.1007/BF01709372 Specific and sensitive two-step polymerase chain reaction assay for the detection of Salmonella species [Elektronische Daten] [W. Haedicke, H. Wolf, W. Ehret, U. Reischl] A polymerase chain reaction (PCR) assay was applied for the selective amplification of a characteristic sequence within aSalmonella-specific chromosomal fragment. A two-temperature PCR cycle enhanced both the speed and overall sensitivity of the amplification procedure. Twenty-one well-characterizedSalmonella strains and a number of non-Salmonella strains were tested. With the exception of the rarely isolatedSalmonella arizonae strain, the PCR-based approach enabled the specific identification ofSalmonella with a detection limit of 103 organisms. In combination with a nested PCR assay, as few as ten organisms were detectable. Specificity was demonstrated as no distinct amplification products were detectable with any of the testednon-Salmonella strains. With a pre-enrichment step using paramagnetic antiSalmonella beads, an increase in sensitivity was observed in the case of clinical samples while the amplification process was not influenced. MMV Medizin Verlag GmbH, 1996 Haedicke W. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut Wolf H. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut Ehret W. Institut für Laboratoriumsmedizin, Zentralklinikum Augsburg, Stenglinstraße, 86156, Augsburg, Germany aut Reischl U. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 603-607 0934-9723 15:7<603 1996 15 10096 https://doi.org/10.1007/BF01709372 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01709372 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haedicke W. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Wolf H. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Ehret W. Institut für Laboratoriumsmedizin, Zentralklinikum Augsburg, Stenglinstraße, 86156, Augsburg, Germany aut NATIONALLICENCE 700 1- Reischl U. Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 603-607 0934-9723 15:7<603 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer