caa a22 4500 477070159 CHVBK 20180405111423.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01709362 doi (NATIONALLICENCE)springer-10.1007/BF01709362 Detection and identification of Mycobacterium avium in the blood of AIDS patients by the polymerase chain reaction [Elektronische Daten] [M. De Francesco, D. Colombrita, G. Pinsi, F. Gargiulo, S. Caligaris, D. Bertelli, F. Martinelli, J. Gao, A. Turano] One hundred fifty-three blood samples from patients positive for the human immunodeficiency virus (HIV) were analyzed by polymerase chain reaction (PCR) to detect the presence ofMycobacterium avium. Samples were collected from patients who also had blood cultures performed by a radiometric method. Blood samples were centrifuged on a Ficoll-Hypaque gradient to purify peripheral blood mononuclear cells. The purified cells were washed and incubated with a resin, boiled to release mycobacterial DNA, and then amplified. Polymerase chain reaction products were detected by a nonisotopic method. A 123 base-pair (bp) insertion sequence, namely IS6110, fromMycobacterium tuberculosis complex was also included in the reaction as an internal control ofTaq polymerase activity to exclude the presence of enzyme inhibitors. This IS6110 fragment can be distinguished from the 383 bp target product on ethidium bromide-stained agarose gel and may also be used in a colorimetric assay. Such results were compared with the results of culture and indicated that the assay is as sensitive as bacteriological methods, though faster. MMV Medizin Verlag GmbH, 1996 De Francesco M. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Colombrita D. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Pinsi G. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Gargiulo F. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Caligaris S. II and I Division of Infectious Diseases, Spedali Civili, Brescia, Italy aut Bertelli D. II and I Division of Infectious Diseases, Spedali Civili, Brescia, Italy aut Martinelli F. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Gao J. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut Turano A. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 551-555 0934-9723 15:7<551 1996 15 10096 https://doi.org/10.1007/BF01709362 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01709362 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Francesco M. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Colombrita D. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Pinsi G. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Gargiulo F. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Caligaris S. II and I Division of Infectious Diseases, Spedali Civili, Brescia, Italy aut NATIONALLICENCE 700 1- Bertelli D. II and I Division of Infectious Diseases, Spedali Civili, Brescia, Italy aut NATIONALLICENCE 700 1- Martinelli F. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Gao J. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 700 1- Turano A. Institute of Microbiology, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/7(1996-07-01), 551-555 0934-9723 15:7<551 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer