caa a22 4500 477070256 CHVBK 20180405111423.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF01690516 doi (NATIONALLICENCE)springer-10.1007/BF01690516 Prevalence of Dientamoeba fragilis antibodies in children and recognition of a 39 kDa immunodominant protein antigen of the organism [Elektronische Daten] [F. Chan, N. Stewart, M. Guan, L Robb, L. Fuite, I. Chan, F. Diaz-Mitoma, J. King, N. MacDonald, A. Mackenzie] Dientamoeba fragilis, a common intestinal protozoan parasite in Canada, has been associated with diarrhoea and abdominal pain in some patients. Seroprevalence of this organism has not been reported previously. In the present study sera from three symptomatic patients, 12 age- and sexmatched controls, and 189 randomly selected healthy individuals (age 6 months to 19 years) were tested for antibodies againstDientamoeba fragilis by an indirect immunofluorescence (IIF) assay. All three symptomatic patients infected withDientamoeba fragilis had positive IIF titres of 80, and all 12 matched controls had positive titres ranging 20 to 160 (geometric mean titre 48). Of the 189 healthy children, 172 (91%) were positive at a serum dilution of 1∶10 or higher. The specificity of the IIF assay was reinforced by immunoblotting 20 representative serum samples againstDientamoeba fragilis, In all 17 IIF-positive serum samples, a 39 kDa protein band ofDientamoeba fragilis was identified, the same band recognized by a mouse monoclonal antibody raised in our laboratory. Findings over a five-year period indicate thatDientamoeba fragilis was the most common protozoan, followed closely byGiardia lamblia and more distantly byCryptosporidium parvum. The high seropositivity of 91% forDientamoeba fragilis compares reasonably well with serologic data obtained by IIF and reported previously forGiardia lamblia (85.6%) andCryptosporidium parvum (86%). Springer-Verlag, 1996 Chan F. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Stewart N. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Guan M. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Robb L. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Fuite L. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Chan I. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Diaz-Mitoma F. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut King J. Department of Pediatrics, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut MacDonald N. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut Mackenzie A. Division of Microbiology, Ottawa Civic Hospital, K1Y 4E9, Ottawa, Ontario, Canada aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/12(1996-12-01), 950-954 0934-9723 15:12<950 1996 15 10096 https://doi.org/10.1007/BF01690516 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01690516 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan F. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Stewart N. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Guan M. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Robb L. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Fuite L. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Chan I. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Diaz-Mitoma F. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- King J. Department of Pediatrics, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- MacDonald N. Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, K1H 8L1, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Mackenzie A. Division of Microbiology, Ottawa Civic Hospital, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/12(1996-12-01), 950-954 0934-9723 15:12<950 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer