caa a22 4500 47707040X CHVBK 20180405111423.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF01690509 doi (NATIONALLICENCE)springer-10.1007/BF01690509 Performance of six culture media for isolation of Salmonella species from stool samples [Elektronische Daten] [J. Ruiz, M. Núñez, I. Lorente, J. Pérez, E. Simarro, J. Gómez] The relative performance of five plating media [Rambach agar; salmonella-shigella (SS) agar, novobiocin-brilliant green-glycerol-lactose (NBGL), modified semisolid Rappaport-Vassiliadis medium (MSRV), andSalmonella Detection and Identification-2 (SM2)] and selenite broth (SB) subcultured in SS agar in the recovery ofSalmonella spp. from 500 human stool specimens was evaluated. On Rambach agar and SS agar, the C8-esterase test was also used for selection of suspicious colonies. Eighty-one samples were positive for salmonellae on at least one of the six media. Sensitivities and specificities of MSRV, SB, NBGL, SS, Rambach agar, and SM2 were 95.1 and 98.1%, 87.6 and 99.8%, 79 and 91.9%, 69.1 and 99.3%, 56.8 and 96.9%, and 54.3 and 92.4%, respectively. There were statistically significant differences between MSRV and NBGL, Rambach agar, SS agar, and SM2 (p < 0.005), between SB and SS agar, Rambach agar, and SM2 (p < 0.05), and between NBGL and SM2 and Rambach agar (p < 0.005). The greatest number of isolates was recovered with MSRV, whose performance surpassed that of enrichment in selenite broth, probably because the subcultures were not repeated on MSRV. This hypothesis is now under investigation. The specificity of each of the five solid media was greater than 90%. Springer-Verlag, 1996 Ruiz J. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut Núñez M. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut Lorente I. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut Pérez J. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut Simarro E. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut Gómez J. Department of Infectious Diseases, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/12(1996-12-01), 922-926 0934-9723 15:12<922 1996 15 10096 https://doi.org/10.1007/BF01690509 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01690509 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruiz J. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 700 1- Núñez M. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 700 1- Lorente I. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 700 1- Pérez J. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 700 1- Simarro E. Laboratory of Microbiology, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 700 1- Gómez J. Department of Infectious Diseases, Hospital Universitario "Virgen de la Arrixaca”, Carretera El Palmar-Cartagena, 30120, Murcia, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/12(1996-12-01), 922-926 0934-9723 15:12<922 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer