caa a22 4500 477070426 CHVBK 20180405111423.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01591357 doi (NATIONALLICENCE)springer-10.1007/BF01591357 Morphologic conversion of Helicobacter pylori from bacillary to coccoid form [Elektronische Daten] [M. Sörberg, M. Nilsson, H. Hanberger, L. Nilsson] The morphologic conversion ofHelicobacter pylori from bacillary to coccoid form was studied by microscopy, viable count on agar plates, and bioluminescence assay of bacterial ATP. When morphologic conversion from bacillary to coccoid form was detected by microscopy, the viable counts and the bacterial ATP decreased. No viable count was found after nine days of incubation, but bacterial ATP was still present. In these cultures in which only the coccoid form ofHelicobacter pylori was present, there was no accumulation of extracellular ATP, indicating no leaky cells. During the transition phase from the bacillary to the coccoid form ofHelicobacter pylori, the addition of fresh medium increased the intracellular ATP 26-fold. The coccoid form ofHelicobacter pylori had a 1000-fold lower ATP level per cell compared to the bacillary form, which indicates a decreased metabolic activity in the coccoid form. Addition of fresh medium to the coccoid cultures from days 9 and 10 increased the ATP level twofold. However, no conversion from coccoid to bacillary form was found in these cultures during prolonged incubation in fresh broth for four weeks. Such conversion needs to be demonstrated before it is proven that the coccoid form ofHelicobacter pylori is responsible for transmission and relapse of infection. Springer-Verlag, 1996 Sörberg M. Department of Infectious Diseases, Danderyd Hospital, S-182 88, Danderyd, Sweden aut Nilsson M. Department of Clinical Microbiology, University Hospital, S-58185, Linköping, Sweden aut Hanberger H. Department of Infectious Diseases, University Hospital, S-58185, Linköping, Sweden aut Nilsson L. Department of Clinical Microbiology, University Hospital, S-58185, Linköping, Sweden aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 216-219 0934-9723 15:3<216 1996 15 10096 https://doi.org/10.1007/BF01591357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01591357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sörberg M. Department of Infectious Diseases, Danderyd Hospital, S-182 88, Danderyd, Sweden aut NATIONALLICENCE 700 1- Nilsson M. Department of Clinical Microbiology, University Hospital, S-58185, Linköping, Sweden aut NATIONALLICENCE 700 1- Hanberger H. Department of Infectious Diseases, University Hospital, S-58185, Linköping, Sweden aut NATIONALLICENCE 700 1- Nilsson L. Department of Clinical Microbiology, University Hospital, S-58185, Linköping, Sweden aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 216-219 0934-9723 15:3<216 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer