caa a22 4500 477070485 CHVBK 20180405111423.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01591358 doi (NATIONALLICENCE)springer-10.1007/BF01591358 Discriminatory power and application of ribotyping of Yersinia enterocolitica O:3 in an epidemiological study [Elektronische Daten] [M. Mendoza, R. Alzugaray, E. Landeras, M. González-Hevia] Ribotyping performed with five restriction endonucleases was used in an attempt to subtypeYersinia enterocolitica serotype O:3 and also as a tool for clonal analysis. DNA from organisms under study (48 isolates from diarrheic human feces, 24 from food, and 5 reference strains) was tested by Southern hybridization using a DNA probe carrying an rRNA operon fromEscherichia coli. Strains were grouped into seven ribotypes by theHindIII restriction endonuclease, into five ribotypes byNciI,BglI, and SalI; and into two ribotypes byEcoRI, resulting in a discrimination index (DI) of 0.37, 0.17, 0.43, 0.13, and 0.03 for the five endonucleases. By combining the results obtained with two or more restriction endonucleases, a further discrimination was registered, the most efficient combination (in terms of discriminatory power vs. cost in work, time, and money) for routine typing beingHindIII-BglI (9 types, DI=0.58). In the clonal analysis, results obtained with the five restriction endonucleases allowed us to define 11 groupings or clonal lines, which showed a remarkable degree of genetic heterogeneity (genetic distance coefficients between 0.03 and 0.73) and were grouped into two major clusters. One cluster included 93% of the strains and eight lines. At least two of the most frequent lines can be considered endemic in Asturias, Spain, because organisms belonging to these lines have been circulating and causing human yersiniosis in recent years and have also been isolated from commercial raw meat products. TwoNciI ribotypes from the series under study (92.2% of strains included in the prevalent cluster) were similar but not identical to ribotypes of O:3 organisms from other geographic areas described in the literature, indicating that the genetic structure of prevalent human pathogens of this serotype is basically clonal. Springer-Verlag, 1996 Mendoza M. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut Alzugaray R. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut Landeras E. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut González-Hevia M. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 220-226 0934-9723 15:3<220 1996 15 10096 https://doi.org/10.1007/BF01591358 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01591358 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mendoza M. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Alzugaray R. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- Landeras E. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut NATIONALLICENCE 700 1- González-Hevia M. Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 220-226 0934-9723 15:3<220 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer