caa a22 4500 477070558 CHVBK 20180405111424.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01591363 doi (NATIONALLICENCE)springer-10.1007/BF01591363 Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy [Elektronische Daten] [A. Marchese, G. Arlet, G. Schito, P. Lagrange, A. Philippen] Thirty-fiveKlebsiella pneumoniae strains isolated during 1993-1994 in intensive care units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination. Springer-Verlag, 1996 Marchese A. Institute of Microbiology, University of Genoa, Viale Benedetto XV, 10, 16132, Genoa, Italy aut Arlet G. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut Schito G. Institute of Microbiology, University of Genoa, Viale Benedetto XV, 10, 16132, Genoa, Italy aut Lagrange P. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut Philippen A. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 245-248 0934-9723 15:3<245 1996 15 10096 https://doi.org/10.1007/BF01591363 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01591363 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marchese A. Institute of Microbiology, University of Genoa, Viale Benedetto XV, 10, 16132, Genoa, Italy aut NATIONALLICENCE 700 1- Arlet G. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut NATIONALLICENCE 700 1- Schito G. Institute of Microbiology, University of Genoa, Viale Benedetto XV, 10, 16132, Genoa, Italy aut NATIONALLICENCE 700 1- Lagrange P. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut NATIONALLICENCE 700 1- Philippen A. Service de Microbiologie, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75475, Paris Cedex 10, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/3(1996-03-01), 245-248 0934-9723 15:3<245 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer