caa a22 4500 47707068X CHVBK 20180405111424.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF01701532 doi (NATIONALLICENCE)springer-10.1007/BF01701532 Interlaboratory comparison of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to samples of amniotic fluid [Elektronische Daten] [E. Guy, H. Pelloux, M. Lappalainen, H. Aspöck, A. Haßl, K. Melby, M. Holberg-Pettersen, E. Petersen, J. Simon, P. Ambroise-Thomas] To investigate the accuracy of the polymerase chain reaction (PCR) method for the detection ofToxoplasma gondii in clinical specimens, aliquots of amniotic fluid to which known amounts ofToxoplasma gondii DNA had been added were tested by five European Centres. Four laboratories were able to detect DNA at levels equivalent to ten tachyzoites or less, including two that detected DNA equivalent to a single parasite. Two laboratories erroneously found one of eight negative control samples to be positive. These findings confirm that the high level of sensitivity associated with the PCR method can be readily achieved under routine laboratory conditions, but they also underscore the potential for both false-positive and false-negative findings to occur. Furthermore, the results confirm the urgent need for an external quality assurance scheme to support laboratories employing PCR in a clinical context for the detection ofToxoplasma gondii. Springer-Verlag, 1996 Guy E. Toxoplasma Reference Unit, Public Health Laboratory, Singleton Hospital, SA2 8QA, Swansea, UK aut Pelloux H. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut Lappalainen M. Department of Virology, University of Helsinki, SF-00014, Finland aut Aspöck H. Department of Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, Kinderspitalgasse 15, A-1095, Vienna, Austria aut Haßl A. Department of Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, Kinderspitalgasse 15, A-1095, Vienna, Austria aut Melby K. Department of Microbiology, Ullevål University Hospital, University of Oslo, N-0407, Oslo, Norway aut Holberg-Pettersen M. Department of Microbiology, Ullevål University Hospital, University of Oslo, N-0407, Oslo, Norway aut Petersen E. Laboratory of Parasitology, Statens Seruminstitut, 5 Artillerivej, DK-2300, Copenhagen S, Denmark aut Simon J. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut Ambroise-Thomas P. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 836-839 0934-9723 15:10<836 1996 15 10096 https://doi.org/10.1007/BF01701532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01701532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guy E. Toxoplasma Reference Unit, Public Health Laboratory, Singleton Hospital, SA2 8QA, Swansea, UK aut NATIONALLICENCE 700 1- Pelloux H. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut NATIONALLICENCE 700 1- Lappalainen M. Department of Virology, University of Helsinki, SF-00014, Finland aut NATIONALLICENCE 700 1- Aspöck H. Department of Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, Kinderspitalgasse 15, A-1095, Vienna, Austria aut NATIONALLICENCE 700 1- Haßl A. Department of Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, Kinderspitalgasse 15, A-1095, Vienna, Austria aut NATIONALLICENCE 700 1- Melby K. Department of Microbiology, Ullevål University Hospital, University of Oslo, N-0407, Oslo, Norway aut NATIONALLICENCE 700 1- Holberg-Pettersen M. Department of Microbiology, Ullevål University Hospital, University of Oslo, N-0407, Oslo, Norway aut NATIONALLICENCE 700 1- Petersen E. Laboratory of Parasitology, Statens Seruminstitut, 5 Artillerivej, DK-2300, Copenhagen S, Denmark aut NATIONALLICENCE 700 1- Simon J. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut NATIONALLICENCE 700 1- Ambroise-Thomas P. Département de Parasitologie-Mycologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Grenoble, Grenoble Cédex 9, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 836-839 0934-9723 15:10<836 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer