caa a22 4500 47707071X CHVBK 20180405111424.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF01701524 doi (NATIONALLICENCE)springer-10.1007/BF01701524 Urine samples as a possible alternative to serum for human immunodeficiency virus antibody screening [Elektronische Daten] [P. Martínez, R. de Lejarazu, J. Eiros, J. De Benito, A. Rodríguez-Torres] The detection of specific antibodies against human immunodeficiency virus (HIV) was tested by dot blot enzyme immunoassay in 95 urine samples from 72 individuals infected with HIV and 23 seronegative individuals. Western blot of paired serum samples from these same individuals was used as the gold standard. The dot blot tested had a sensitivity of 97.2% and a specificity of 100%; only two samples from HIV-infected individuals at Centers for Disease Control (CDC) stages II and IV were non-reactive. Reactive and discrepant samples (serum/urine) were confirmed by Western blot, which had a sensitivity of 98.6% and a specificity of 100%. The most commonly observed Western blot reactivity pattern in urine samples included bands against three groups of HIV structural proteins (ENV, POL, and GAG). The results indicate that urine can be used in screening for HIV antibodies in epidemiological studies of high-prevalence populations, though it is not recommended for individualized diagnostic purposes. Springer-Verlag, 1996 Martínez P. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut de Lejarazu R. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut Eiros J. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut De Benito J. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut Rodríguez-Torres A. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 810-813 0934-9723 15:10<810 1996 15 10096 https://doi.org/10.1007/BF01701524 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01701524 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez P. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- de Lejarazu R. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Eiros J. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- De Benito J. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Rodríguez-Torres A. Microbiology Laboratory Service, University Hospital, c/ Ramón y Cajal s. n., 47011, Valladolid, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 810-813 0934-9723 15:10<810 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer