caa a22 4500 477070809 CHVBK 20180405111424.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF01701525 doi (NATIONALLICENCE)springer-10.1007/BF01701525 PCR detection of Mycobacteraemia in Tanzanian patients with extrapulmonary tuberculosis [Elektronische Daten] [C. Richter, L. Kox, J. van Leeuwen, I. Mtoni, A. Kolk] In 191 Tanzanian patients admitted to hospital with suspected extrapulmonary tuberculosis (TB), TB was diagnosed in 158 patients; the remaining 33 patients had neither microbiological nor clinical evidence of TB.Mycobacterium tuberculosis was detected in the blood of 25 patients, in 92% by a polymerase chain reaction (PCR) technique and in 52% by culture of buffy coat cells. The presence of mycobacterial DNA orMycobacterium tuberculosis bacteria in peripheral blood (positive culture) was significantly associated with HIV infection; it was detected in 22 (21.4%) of 103 HIV-seropositive patients compared to only 3 (3.5%) of 55 HIV-seronegative patients (p<0.009). In two-thirds of the patients with mycobacteraemia, TB can be detected by simple smears from other organ sites. In patients with suspected extrapulmonary tuberculosis in whom smears from the infected site are negative or not available, PCR on blood will confirm the diagnosis within 24 hours in one third of the cases. Springer-Verlag, 1996 Richter C. Department of Medicine, Muhimbili Medical Centre, Dar es Salaam, Tanzania aut Kox L. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut van Leeuwen J. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut Mtoni I. National Institute for Medical Research, Dar es Salaam, Tanzania aut Kolk A. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 813-817 0934-9723 15:10<813 1996 15 10096 https://doi.org/10.1007/BF01701525 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01701525 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Richter C. Department of Medicine, Muhimbili Medical Centre, Dar es Salaam, Tanzania aut NATIONALLICENCE 700 1- Kox L. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Leeuwen J. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Mtoni I. National Institute for Medical Research, Dar es Salaam, Tanzania aut NATIONALLICENCE 700 1- Kolk A. Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/10(1996-10-01), 813-817 0934-9723 15:10<813 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer