caa a22 4500 477070841 CHVBK 20180405111425.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01695662 doi (NATIONALLICENCE)springer-10.1007/BF01695662 Evaluation of the ability of a commercial system to identify Acinetobacter genomic species [Elektronische Daten] [A. Bernards, J. van der Toorn, C. van Boven, L. Dijkshoorn] A collection of 130Acinetobacter strains identified by DNA hybridization to 18 different genomic species was used to assess the ability of the API 20NE system (bioMérieux, France) to identifyAcinetobacter genomic species and to determine its accuracy. Fiftyeight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. TheAcinetobacter baumannii strains and theAcinetobacter haemolyticus strains were all identified correctly. Three of fiveAcinetobacter junii strains, three of eightAcinetobacter johnsonii strains, and 11 of 13Acinetobacter Iwoffii strains were also identified correctly. The 58 correctly identified strains represented 45% of the total 130 strains. Thirty-six of the 72 inappropriately identified strains were designatedAcinetobacter baumannii. Thirty-one of these 36 strains belonged to genomic species 1 (Acinetobacter calcoaceticus), 3, or 13TU. Analysis of the profiles showed that the API system does not discriminate between genomic species 1, 2, 3, and 13TU. Lumping of these groups into theAcinetobacter calcoaceticus-Acinetobacter baumannii complex in the API 20NE database would make the system considerably more accurate. Incorporation of these data into the database may improve identification of the remaining genomic species, including some that are not defined. However, the discriminative power of the tests in the API galleries is insufficient for correct identification of allAcinetobacter genomic species. Springer-Verlag, 1996 Bernards A. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut van der Toorn J. Kluyver Laboratory for Biotechnology, University of Technology, Julianalaan 67, Delft, The Netherlands aut van Boven C. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut Dijkshoorn L. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 303-308 0934-9723 15:4<303 1996 15 10096 https://doi.org/10.1007/BF01695662 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01695662 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernards A. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut NATIONALLICENCE 700 1- van der Toorn J. Kluyver Laboratory for Biotechnology, University of Technology, Julianalaan 67, Delft, The Netherlands aut NATIONALLICENCE 700 1- van Boven C. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut NATIONALLICENCE 700 1- Dijkshoorn L. Department of Medical Microbiology, Laboratory of Bacteriology, University Hospital Leiden, P.O. Box 9600, 2300, RC Leiden, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 303-308 0934-9723 15:4<303 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer