caa a22 4500 477070876 CHVBK 20180405111425.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01695664 doi (NATIONALLICENCE)springer-10.1007/BF01695664 Molecular characterization of Borrelia burgdorferi sensu lato from Slovenia revealing significant differences between tick and human isolates [Elektronische Daten] [R. Picken, Y. Cheng, F. Strle, J. Cimperman, V. Maraspin, S. Lotric-Furlan, E. Ruzic-Sabljic, D. Han, J. Nelson, M. Picken, G. Trenholme] One hundred twenty-nine Slovenian isolates ofBorrelia burgdorferi sensu lato derived from patients (69 strains) orIxodes ricinus ticks (60 strains) were characterized. All of the strains were first- or second-passage isolates obtained in 1992 and 1993 from the same endemic region. The techniques used for the molecular analysis of strains included species-specific polymerase chain reaction (PCR) typing, and pulsed-field gel electrophoretic separation of undigested andMlul-digested genomic DNA. Isolates were identified to the species level by large restriction fragment pattern (LRFP) analysis and the results compared with the species-specific PCR result. Fifty-two patient isolates (75%) were typed asBorrelia afzelii (LRFP MLa1), 6 (9%) asBorrelia garinii (LRFPs MLg1-4), and 11 (16%) asBorrelia burgdorferi sensu stricto. The latter included 9 isolates (13%) with a new LRFP that is not typical ofBorrelia burgdorferi sensu stricto and for which the designation MLx is suggested. In contrast, only 32 of 60 (53%) tick isolates were typed asBorrelia afzelii, while 20 strains (33%) were typed asBorrelia garinii and 8 strains (13%) asBorrelia burgdorferi sensu stricto. Three new LRFPs were found among theBorrelia garinii (MLg5 and 6) andBorrelia burgdorferi sensu stricto (MLb15) tick isolates. Large restriction fragment pattern analysis identified new groups ofBorrelia burgdorferi sensu lato and revealed an apparent difference in the isolation frequency of different species from patients and ticks in the same endemic region. Springer-Verlag, 1996 Picken R. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut Cheng Y. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut Strle F. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut Cimperman J. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut Maraspin V. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut Lotric-Furlan S. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut Ruzic-Sabljic E. Institute of Microbiology, University of Ljubljana, Zaloska 4, 61105, Ljubljana, Slovenia aut Han D. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut Nelson J. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut Picken M. Department of Pathology, Building 110, Room 2242, Loyola University Medical Center, 2160 South First Avenue, 60153, Maywood, IL aut Trenholme G. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 313-323 0934-9723 15:4<313 1996 15 10096 https://doi.org/10.1007/BF01695664 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01695664 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Picken R. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut NATIONALLICENCE 700 1- Cheng Y. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut NATIONALLICENCE 700 1- Strle F. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Cimperman J. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Maraspin V. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Lotric-Furlan S. Department of Infectious Diseases, University Medical Centre, Japljeva 2, 61000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Ruzic-Sabljic E. Institute of Microbiology, University of Ljubljana, Zaloska 4, 61105, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Han D. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut NATIONALLICENCE 700 1- Nelson J. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut NATIONALLICENCE 700 1- Picken M. Department of Pathology, Building 110, Room 2242, Loyola University Medical Center, 2160 South First Avenue, 60153, Maywood, IL aut NATIONALLICENCE 700 1- Trenholme G. Section of Infectious Disease, Rush-Presbyterian-St. Luke's Medical Center, 600 South Paulina, 60612, Chicago, IL, USA aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 313-323 0934-9723 15:4<313 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer