caa a22 4500 477070922 CHVBK 20180405111425.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01695661 doi (NATIONALLICENCE)springer-10.1007/BF01695661 Rapid genotyping of Escherichia coli O157 isolates by random amplification of polymorphic DNA [Elektronische Daten] [M. Birch, D. Denning, D. Law] Although several typing methods have been described for Shiga-like toxin-producingEscherichia coli O157, the methods are somewhat cumbersome. Using 22 isolates ofEscherichia coli O157 and five otherEscherichia coli isolates, two primers (M13 core sequence and 970-11) were found to give excellent differentiation between isolates using random amplified polymorphic DNA (RAPD). Using only the presence or absence of variable bands, a matrix of 20 variable characters was identified. From these characters, similarity coefficients were calculated and a phenogram constructed. All of theEscherichia coli O157 isolates were easily distinguished from the non-O157Escherichia coli isolates. Using a 95% similarity cutoff, we found 13 RAPD types among the 22Escherichia coli O157 isolates. Isolates thought to be identical by toxin and phage typing as well as by epidemiological association were distinguished, and others thought to be distinct by lack of epidemiological association were identical. RAPD using M13 and 970-11 primers is a potentially useful typing tool forEscherichia coli isolates of serotype O157 and possibly otherEscherichia coli isolates. Springer-Verlag, 1996 Birch M. Department of Medicine, University of Manchester, UK aut Denning D. Department of Medicine, University of Manchester, UK aut Law D. Department of Microbiology, Hope Hospital, Stott Lane, M6 8HD, Salford, UK aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 297-302 0934-9723 15:4<297 1996 15 10096 https://doi.org/10.1007/BF01695661 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01695661 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Birch M. Department of Medicine, University of Manchester, UK aut NATIONALLICENCE 700 1- Denning D. Department of Medicine, University of Manchester, UK aut NATIONALLICENCE 700 1- Law D. Department of Microbiology, Hope Hospital, Stott Lane, M6 8HD, Salford, UK aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/4(1996-04-01), 297-302 0934-9723 15:4<297 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer