caa a22 4500 477071333 CHVBK 20180405111426.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01690093 doi (NATIONALLICENCE)springer-10.1007/BF01690093 Influence of oropharyngeal flora and specimen pretreatment on the recovery of Helicobacter pylori [Elektronische Daten] [D. Jonkers, E. Stobberingh, R. Stockbrügger] The recovery ofHelicobacter pylori was studied in vitro in the presence of different concentrations of oropharyngeal flora. The effect on the bacterial yield ofHelicobacter pylori and oropharyngeal flora of washing biopsies once, twice or thrice before culture was then studied in biopsies taken from 35 patients. A low rate of recovery of lowHelicobacter pylori concentrations (< 103 cfu/ml) was related to the presence of relatively high oropharyngeal flora concentrations (≥ 104 cfu/ml). After three washings a decrease in oropharyngeal flora between 10-fold and 100-fold cfu/biopsy was observed in seven patient samples. In another three specimens a more than 100-fold reduction in cfu/biopsy was observed. In the case ofHelicobacter pylori, a 60-fold decrease in log cfu/biopsy was observed in only one patient. Pretreatment of the specimens by washing of the biopsies improved the recovery of small amounts ofHelicobacter pylori in 10 of 28 patients. For optimal detection ofHelicobacter pylori in gastric mucosal biopsies, washing of the specimens in addition to the use of selective media is recommended. Springer-Verlag, 1996 Jonkers D. Department of Gastroenterology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut Stobberingh E. Department of Microbiology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut Stockbrügger R. Department of Gastroenterology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/5(1996-05-01), 378-382 0934-9723 15:5<378 1996 15 10096 https://doi.org/10.1007/BF01690093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01690093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jonkers D. Department of Gastroenterology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut NATIONALLICENCE 700 1- Stobberingh E. Department of Microbiology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut NATIONALLICENCE 700 1- Stockbrügger R. Department of Gastroenterology, Academic Hospital Maastricht, P. Debeyelaan 25, P.O. Box 5800, 6202, AZ Maastricht, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/5(1996-05-01), 378-382 0934-9723 15:5<378 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer