caa a22 4500 47707135X CHVBK 20180405111426.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01690097 doi (NATIONALLICENCE)springer-10.1007/BF01690097 Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test [Elektronische Daten] [M. Nüesch-Inderbinen, H. Hächler, F. Kayser] A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum β-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of thebla SHV genes, followed by restriction withNheI. Due to the glycine (position 238) (SHV-non-ESBL) → serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carryingbla SHV-ESBL genes as ESBL producers. Springer-Verlag, 1996 Nüesch-Inderbinen M. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut Hächler H. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut Kayser F. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/5(1996-05-01), 398-402 0934-9723 15:5<398 1996 15 10096 https://doi.org/10.1007/BF01690097 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01690097 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nüesch-Inderbinen M. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut NATIONALLICENCE 700 1- Hächler H. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut NATIONALLICENCE 700 1- Kayser F. Department of Medical Microbiology, University of Zürich, PO Box, CH-8028, Zürich, Switzerland aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/5(1996-05-01), 398-402 0934-9723 15:5<398 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer