caa a22 4500 47707149X CHVBK 20180405111427.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01591484 doi (NATIONALLICENCE)springer-10.1007/BF01591484 Comparative analysis of six European influenza vaccines [Elektronische Daten] [I. Chaloupka, A. Schuler, M. Marschall, H. Meier-Ewert] Three split-virion vaccines (Vaxigrip, Begrivac, and Influsplit/Fluarix) and three subunit vaccines containing only viral surface glycoproteins (Influvac, Agrippai, and Fluvirin) available for the 1994-95 season were analysed by biological, molecular, and biochemical methods. Although all vaccines are required by health authorities to contain 15 μg haemagglutinin per dose of each virus strain, there were significant differences in haemagglutination titres among the examined vaccines of both types. The enzymatic activity of neuraminidase was present in all vaccines except Fluvirin. Total protein content was lower for subunit vaccines. Viral nucleoprotein was detected in all split vaccines but to varying levels according to SDS-PAGE and Western blot analyses. The ovalbumin content was low in general but was about tenfold higher for Influvac than for the other vaccines analysed. This protein may induce hypersensitive reactions among persons with severe egg allergy. All three split-virion vaccines were found to contain the matrix protein; however, it was not detected in the subunit vaccines. Differences in influenza antigen variety in currently available vaccines may affect efficacy, whereas differences in concentrations of nonviral compounds such as ovalbumin and endotoxin may lead to different postvaccination reactogenicity profiles. MMV Medizin Verlag GmbH Munchen, 1996 Chaloupka I. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut Schuler A. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut Marschall M. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut Meier-Ewert H. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/2(1996-02-01), 121-127 0934-9723 15:2<121 1996 15 10096 https://doi.org/10.1007/BF01591484 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01591484 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaloupka I. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut NATIONALLICENCE 700 1- Schuler A. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut NATIONALLICENCE 700 1- Marschall M. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut NATIONALLICENCE 700 1- Meier-Ewert H. Abteilung für Virologie, Institut für Medizinische Mikrobiologie der Technische Universität München, Biedersteiner Str. 29, 80802, Munich, Germany aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/2(1996-02-01), 121-127 0934-9723 15:2<121 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer