caa a22 4500 477071724 CHVBK 20180405111427.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF01691225 doi (NATIONALLICENCE)springer-10.1007/BF01691225 Detection of hepatitis C virus RNA in serum and peripheral blood mononuclear cells in patients with chronic hepatitis C treated with interferon alpha [Elektronische Daten] [M. Basaras, B. de las Heras, M. García Bengoechea, L. Gallego, E. Arrese, R. Cisterna] PCR was used to detect hepatitis C virus (HCV) RNA in serum and peripheral blood mononuclear cells (PBMCs) for evaluation of a six-month course of Interferon therapy in 18 patients with histologically confirmed chronic hepatitis C. At follow-up six months after the end of therapy positive-stranded (genomic) and negative-stranded (anti-genomic, presumptive replicative intermediate) HCV RNA could be detected in PBMCs of all ten patients who either did not respond to therapy or suffered a relapse; genomic strand RNA was detected in five patients who responded but then relapsed. The study confirms that interferon therapy leads to inhibition of HCV replication but not eradication of the virus. Persistence of the virus at extrahepatic sites may explain its reactivation after cessation of interferon therapy. Springer-Verlag, 1996 Basaras M. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut de las Heras B. Department of Medicine, Hospital de Basurto, Bilbao, Spain aut García Bengoechea M. Department of Medicine, Hospital de Nuestra Senora de Aránzazu, San Sebastian, Spain aut Gallego L. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut Arrese E. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut Cisterna R. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/11(1996-11-01), 887-890 0934-9723 15:11<887 1996 15 10096 https://doi.org/10.1007/BF01691225 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01691225 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Basaras M. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut NATIONALLICENCE 700 1- de las Heras B. Department of Medicine, Hospital de Basurto, Bilbao, Spain aut NATIONALLICENCE 700 1- García Bengoechea M. Department of Medicine, Hospital de Nuestra Senora de Aránzazu, San Sebastian, Spain aut NATIONALLICENCE 700 1- Gallego L. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut NATIONALLICENCE 700 1- Arrese E. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut NATIONALLICENCE 700 1- Cisterna R. Department of Microbiology, School of Medicine, Universidad del País Vasco, Apto. 699, 48080, Bilbao, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology and Infectious Diseases Springer-Verlag 15/11(1996-11-01), 887-890 0934-9723 15:11<887 1996 15 10096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer