caa a22 4500 477072038 CHVBK 20180405111428.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02627958 doi (NATIONALLICENCE)springer-10.1007/BF02627958 Protection from preconditioning can be reinstated at various reperfusion intervals [Elektronische Daten] [Efstathios Iliodromitis, Constantinos Papadopoulos, Ioannis Paraskevaidis, Zenon Kyriakides, Constantina Flessa, Dimitrios Kremastinos] Summary: The aim of this study was to evaluate the way in which short-term protection declines and is eventually lost in preconditioning and to determine the efficacy of a second preconditioning at various reperfusion intervals. Male rabbits were divided into six groups. Forty-five minutes (sustained) ischemia followed by 120 minutes reperfusion was applied 60, 65, 70, 75, and 80 minutes after a 5 minute preconditioning (groups A, B, C, D, and E) and in a control group (F) after no preconditioning. The infarct to risk ratio (I/R) was 38.3±3.5% in group A, 46.0±7.8% in B, 61.6±9.7% in C, 68.1±4.2% in D, 64.5±7.8% in E, and 61.0±7.7% in F. Group A had a smaller I/R compared with groups C, D, E. and F (p<0.05). In another series, groups G, H, and I were exposed to two 5-minute preconditioning stimuli, separated, respectively, by 45, 60, and 75 minutes of reperfusion; 10 minutes after the last preconditioning, the animals were exposed to 45-minutes ischemia and 120 minutes reperfusion. Groups A and D (with the smaller and higher I/R ratio) were also incorporated into this protocol in order to compare the effect of the additional preconditioning with the single one. The I/R ratio was 25.4±8.5% in group G, 22.8±7.0% in group H, and 14.7±4.0% in group I (p=NS). Group D showed a higher I/R compared with groups G, A, and H (p <0.01), and group I had a smaller I/R compared with groups A (p<0.01) and D (p<0.001). Cardioprotection after a first preconditioning declines gradually and is eventually lost. An additional preconditioning is always effective, and the longer the interval from the first preconditioning, the more potent is the effect. Kluwer Academic Publishers, 1996 preconditioning nationallicence ischemia nationallicence protection nationallicence reintroduction nationallicence Iliodromitis Efstathios 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Papadopoulos Constantinos 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Paraskevaidis Ioannis 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Kyriakides Zenon 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Flessa Constantina 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Kremastinos Dimitrios 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 341-346 0920-3206 10:3<341 1996 10 10557 https://doi.org/10.1007/BF02627958 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02627958 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iliodromitis Efstathios 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 700 1- Papadopoulos Constantinos 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 700 1- Paraskevaidis Ioannis 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 700 1- Kyriakides Zenon 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 700 1- Flessa Constantina 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 700 1- Kremastinos Dimitrios 2nd Department of Cardiology, Onassis Cardiac Surgery Center, Athens, Greece aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 341-346 0920-3206 10:3<341 1996 10 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer