caa a22 4500 477072097 CHVBK 20180405111429.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02627957 doi (NATIONALLICENCE)springer-10.1007/BF02627957 EUK-8 a synthetic catalytic scavenger of reactive oxygen species protects isolated iron-overloaded rat heart from functional and structural damage induced by ischemia/reperfusion [Elektronische Daten] [S. Pucheu, F. Boucher, T. Sulpice, N. Tresallet, Y. Bonhomme, B. Malfroy, J. de Leiris] Summary: The effects of EUK-8, a synthetic, catalytic scavenger of reactive oxygen species, on isolated iron-overloaded rat hearts submitted to ischemia-reperfusion were studied. In the absence of EUK-8, functional parameters (systolic and diastolic pressures, oxygen consumption as estimated by the product heart rate times left ventricular diastolic pressure) were severely impaired 1 minute and 15 minutes after reperfusion following a 15 minute ischemic episode. Dimethylthiourea (10 mM), a hydroxyl radical scavenger, had a minimally protective effect. In contrast, EUK-8 at a concentration of 50 μM in the perfusion medium maintained these parameters at close to their preischemia values. Electron microscopic analysis of heart tissues after 15 minutes ischemia followed by 15 minutes reperfusion showed extensive damage to mitochondria and sarcomeres in untreated hearts, while the extent of damage was significantly lower in EUK-8-treated hearts. The functional and structural protection afforded by EUK-8 were significantly better than those induced by dimethylthiourea. These data suggest that EUK-8 may be therapeutically useful in preventing heart damage induced by ischemia-reperfusion, for example, during thrombolytic treatment of myocardial infarction. Kluwer Academic Publishers, 1996 ischemia-reperfusion nationallicence oxygen free radicals nationallicence isolated rat heart nationallicence iron overload nationallicence EUK-8 nationallicence Pucheu S. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut Boucher F. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut Sulpice T. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut Tresallet N. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut Bonhomme Y. Lipha, s.a., Lyon, France aut Malfroy B. Eukarion, Bedford, MA, USA aut de Leiris J. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 331-339 0920-3206 10:3<331 1996 10 10557 https://doi.org/10.1007/BF02627957 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02627957 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pucheu S. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Boucher F. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Sulpice T. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Tresallet N. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 700 1- Bonhomme Y. Lipha, s.a., Lyon, France aut NATIONALLICENCE 700 1- Malfroy B. Eukarion, Bedford, MA, USA aut NATIONALLICENCE 700 1- de Leiris J. Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 331-339 0920-3206 10:3<331 1996 10 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer