caa a22 4500 477072151 CHVBK 20180405111429.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02627963 doi (NATIONALLICENCE)springer-10.1007/BF02627963 Effect of dietary supplementation with fish oil on cyclosporine A-induced vascular toxicity [Elektronische Daten] [Guy Berkenboom, Dmitri Brékine, Philippe Unger, Myriam Richelle, Yvon Carpentier, Jeanine Fontaine] Summary: We wished to determine whether dietary supplementation with fish oil prevents the vascular toxicity of cyclosporine (Cx). In a first set of experiments, we assessed the endothelial function of aortas isolated from rats supplevage, and providing 150 mg/kg/day of eicosapentaenoic acid and 100 mg/kg/day of docosahexaenoic acid. FO treatment altered neither acetylcholine- and histamine-induced relaxations, nor serotonin-induced contractions (NS vs. control group). Thereafter, three groups of rats were treated in parallel. Group 1 received FO supplementation (by gavage) for 6 weeks, and Cx (10 mg/kg/day po) was added during the last 2 weeks, group 2 received Cx only (10 mg/kg/day po) for 2 weeks, and group 3 served as a control. Both acetylcholine-and histamine-induced relaxations were reduced in group 2 compared with the control group, as indicated by the area under the curve (AUC), which was significantly higher: 296 ±17 vs. 138±32, and 392±38 vs. 318±25 for acetylcholine and histamine, respectively. In group 1, AUC for acetylcholine remained significantly different from the control (241±31 vs. 138±32), whereas AUC for histamine was 367±28 (NS vs. control). The serotonin-induced contractions were also enhanced in group 2 compared with those of the control group, and this alteration was not attenuated in group 1. After mechanical removal of the endothelium the increased responsiveness to serotonin persisted in groups 1 and 2, suggesting this functional alteration to be located in the smooth muscle cells. Thus, in the rat the attenuation of Cx-induced vascular toxicity by fish oil supplementation is only partial, that is, it involves a slight improvement in endothelial function, but with persistence of functional changes in smooth muscle. Kluwer Academic Publishers, 1996 cyclosporine nationallicence fish oil nationallicence rat aorta nationallicence Berkenboom Guy Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut Brékine Dmitri Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut Unger Philippe Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut Richelle Myriam Department of Experimental Surgery, Erasme Hospital, Erasme, Brussels, Belgium aut Carpentier Yvon Department of Experimental Surgery, Erasme Hospital, Erasme, Brussels, Belgium aut Fontaine Jeanine Department of Pharmacology,Institute of Pharmacy, Université Libre de Bruxelles, Brussels, Belgium aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 379-385 0920-3206 10:3<379 1996 10 10557 https://doi.org/10.1007/BF02627963 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02627963 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berkenboom Guy Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut NATIONALLICENCE 700 1- Brékine Dmitri Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut NATIONALLICENCE 700 1- Unger Philippe Department of Cardiology, Erasme Hospital, Erasme, Brussels, Belgium aut NATIONALLICENCE 700 1- Richelle Myriam Department of Experimental Surgery, Erasme Hospital, Erasme, Brussels, Belgium aut NATIONALLICENCE 700 1- Carpentier Yvon Department of Experimental Surgery, Erasme Hospital, Erasme, Brussels, Belgium aut NATIONALLICENCE 700 1- Fontaine Jeanine Department of Pharmacology,Institute of Pharmacy, Université Libre de Bruxelles, Brussels, Belgium aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/3(1996-07-01), 379-385 0920-3206 10:3<379 1996 10 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer