caa a22 4500 477072526 CHVBK 20180405111430.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00050996 doi (NATIONALLICENCE)springer-10.1007/BF00050996 Cytotoxicity of amiodarone in cultured human endothelial cells [Elektronische Daten] [Bruno Baudin, Bénédicte Bénéteau-Burnat, Jacqueline Giboudeau] Summary: As damage to the pulmonary vascular endothelium may be responsible for the lung toxicity of amiodarone, we evaluated the cytolytic toxicity of the drug in cultures of endothelial cells. Cells were cultured from human umbilical cord veins. Amiodarone caused a vacuolization of the cells with liberation of both lactate dehydrogenase (LDH) and angiotensin-converting enzyme (ACE) in the culture medium. These effects were both concentration and time dependent, and were correlated between them. The first toxic effects were shown as soon as 2 hours after contact with the drug and at 0.1 mg/ml, a concentration that can be reached in plasma of amiodarone-treated patiens. A decrease of ACE activity in the cells was delayed to 24 hours and only with the 10 mg/ml concentration. This event correlated with cell death and detachment from the extracellular matrix. LDH increases corresponded to its isoenzymes 3 and 4. These data support the hypothesis of a direct toxic effect of amiodarone on the endothelium and show the need for evaluating LDH, total activity and isoenzymic profile, and ACE determinations in the plasma of patients treated with amiodarone for ischemic heart disease or arrhythmia. Kluwer Academic Publishers, 1996 amiodarone nationallicence endothelium nationallicence angiotensin I-converting enzyme nationallicence lung toxicity nationallicence LDH isoenzymes nationallicence Baudin Bruno Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut Bénéteau-Burnat Bénédicte Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut Giboudeau Jacqueline Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/5(1996-11-01), 557-560 0920-3206 10:5<557 1996 10 10557 https://doi.org/10.1007/BF00050996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00050996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baudin Bruno Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Bénéteau-Burnat Bénédicte Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut NATIONALLICENCE 700 1- Giboudeau Jacqueline Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris, France aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 10/5(1996-11-01), 557-560 0920-3206 10:5<557 1996 10 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer