caa a22 4500 477073247 CHVBK 20180405111432.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02765796 doi (NATIONALLICENCE)springer-10.1007/BF02765796 Production of Paecilomyces Fumosoroseus conidia in submerged culture [Elektronische Daten] [M. de la Torre, H. Cárdenas-Cota] Production de conidies de Paecilomyces fumosoroseus en culture immergée Mexican isolates ofPaecilomyces fumosoroseus (Wize) Brown & Smith virulent to nymphs and adults ofBemisia tabaci Gennadius (Homoptera: Aleyrodidae) were screened in terms of spore production in submerged culture. Effects of light, temperature stress and yeast extract on sporulation were studied. Cycles of 12 hours light/12 hours dark increased spore production as well as an incubation for 24 hours at 37°C prior to incubation at 30°C. In absence of organic nitrogen both fungal growth and sporulation were very low. Spore production in fermentors with a culture media of a C:N ratio of 25 was doubled as compared to a media with a C:N ratio of 11. Both conidia and blastospores were produced. Production of conidia directly from blastospores through microcyclic sporulation was observed. The proportion of conidia obtained under optimal conditions was 88.8%. Submerged culture ofP. fumosoroseus seemed advantageous compared to ricefilled plastic bags production method because of shorter fermentation times, higher spore yields and substantially higher volumetric spore productivity. Results indicated that careful manipulation of nutritional and environmental conditions allowed for production of conidia during submerged growth ofP. fumosoroseus, microcyclic sporulation being induced under a set of environmental conditions including temperature stress and nutrients limitation. Lavoisier Abonnements, 1996 submerged conidiation nationallicence microcyclic sporulation nationallicence spore massproduction nationallicence Bemisia tabaci nationallicence microbial control nationallicence de la Torre M. Department of Biotechnology, CINVESTAV-IPN, P.O.Box 14-740, 07000, México City, México aut Cárdenas-Cota H. Department of Biotechnology, CINVESTAV-IPN, P.O.Box 14-740, 07000, México City, México aut Entomophaga Springer Netherlands 41/3-4(1996-09-01), 443-453 0013-8959 41:3-4<443 1996 41 10526 https://doi.org/10.1007/BF02765796 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02765796 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de la Torre M. Department of Biotechnology, CINVESTAV-IPN, P.O.Box 14-740, 07000, México City, México aut NATIONALLICENCE 700 1- Cárdenas-Cota H. Department of Biotechnology, CINVESTAV-IPN, P.O.Box 14-740, 07000, México City, México aut NATIONALLICENCE 773 0- Entomophaga Springer Netherlands 41/3-4(1996-09-01), 443-453 0013-8959 41:3-4<443 1996 41 10526 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer