caa a22 4500 477074170 CHVBK 20180405111435.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02252856 doi (NATIONALLICENCE)springer-10.1007/BF02252856 Mechanism of action of nitric oxide donors on voltage-activated calcium channels in vascular smooth muscle cells [Elektronische Daten] [A. Zima, A. Belevich, A. Povstyan, M. Kharkhun, Ya. Tsitsyura, M. Shuba] The effects of nitric oxide (NO) donors on inward barium current (I Ba) in freshly isolated smooth muscle cells (SMC) of the guinea pig mesenteric artery and on inward calcium current (I Ca) in SMC of the canine coronary artery were studied using a patch-clamp recording technique in whole-cell configuration. The inward current in SMC of the guinea pig artery was shown to flow through a single type of calcium channels, which have characteristics of high-threshold slowly inactivated channels of L-type. Nitroglycerin (NG) and sodium nitroprusside (NP) reversibly inhibitedI Ba in a dose-dependent manner. Effects of NO donors onI Ba were related to the changes in voltage-dependent properties of calcium channels. In particular, NG and NP accelerated the current inactivation, and their blocking effects increased with the membrane depolarization. Methylene blue, the guanylate cyclase inhibitor, decreased the inhibitory action of NG onI Ba by a factor of 5. 8-Bromo-cGMP, the membrane-permeant cGMP analog, evokedI Ba inhibition similar to that caused by NO donors. In the canine coronary artery, NO donors also inhibitedI Ca flowing through the L-type calcium channels. It has been concluded that NO originating from NG and NP inhibits activity of L-type calcium channels in vascular SMC; it is possible that cGMP-dependent processes are involved. Plenum Publishing Corporation, 1997 Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Belevich A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Povstyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Kharkhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Tsitsyura Ya Shevchenko State University, Kiev, Ukraine aut Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/6(1996-11-01), 232-239 0090-2977 28:6<232 1996 28 11062 https://doi.org/10.1007/BF02252856 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02252856 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Belevich A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Povstyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Kharkhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Tsitsyura Ya Shevchenko State University, Kiev, Ukraine aut NATIONALLICENCE 700 1- Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/6(1996-11-01), 232-239 0090-2977 28:6<232 1996 28 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer