caa a22 4500 477074294 CHVBK 20180405111435.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02262766 doi (NATIONALLICENCE)springer-10.1007/BF02262766 Effect of dalargin on the activity of cytochrome oxidase and glutamate dehydrogenase in rat spinal cord dissociated cultured neurons [Elektronische Daten] [V. Goncharuk, V. Kalenchuk] Dissociated cultured neurons from the rat embryo spinal cord were grown for six days in the presence of dalargin, the synthetic analog of leu-enkephalin. Then the activities of two enzymes of energy metabolism, cytochrome oxidase (CO) and glutamate dehydrogenase (GDH), were studied in these neurons using quantitative cytochemical technique. Dalargin, which possesses the properties of nerve growth factor, enhanced the nerve cell growth and increased the activity of the above enzymes, with GDH activity being increased more significantly. According to the classical standpoint, increased GDH activity under conditions of acute energy deflciency favors the invoivement of some amino acids in a citric acid cycle for subsequent reproduction. One can suggest, in this relation, that the increased energy production caused by the enhanced nerve cell growth in the presence of dalargin was partially compensated by the amino acid splitting. The results allow us to suggest that the effect of dalargin (growth factor) on the nerve cells is similar to the effects of the extremal factors, and requires additional energy to be supplied. Plenum Publishing Corporation, 1997 Goncharuk V. Cardiological Scientific Center, Russian Academy of Medical Sciences, Moscow, Russia aut Kalenchuk V. Cardiological Scientific Center, Russian Academy of Medical Sciences, Moscow, Russia aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/2-3(1996-03-01), 73-76 0090-2977 28:2-3<73 1996 28 11062 https://doi.org/10.1007/BF02262766 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02262766 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goncharuk V. Cardiological Scientific Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Kalenchuk V. Cardiological Scientific Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/2-3(1996-03-01), 73-76 0090-2977 28:2-3<73 1996 28 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer