caa a22 4500 477074332 CHVBK 20180405111435.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02262767 doi (NATIONALLICENCE)springer-10.1007/BF02262767 ATP-induced currents in submucous plexus neurons of the guinea pig small intestine [Elektronische Daten] [A. Glushakov, A. Melishchuk, V. Skok] ATP-induced membrane durrents in the submucous neurons of the guinea pig small intestine were studied using the whole-cell patch-clamp recording technique. Being applied at −50 mV. ATP activated an inward non-selective cationic current in 68.3% of the investigated neurons. An increase in ATP concentration within the 1-1,000 µM range resulted in the s-like increase in the amplitude of ATP-induced current. The EC50 was 150.0±18.5 µM, while the Hill number was 1.6. The current was selectively activated by ATP and was not blocked by P2 purinoreceptor antagonist suramin (50-300 µM).α,β-Methylene-ATP (100-200 µM) andβ,γ-methylene-ATP (100-200µM), which are P2-purinoreceptor agonists, as well as adenosine (100-300 µM), exerted no effects. Reactive blue 2, if applied up to 4 min, enhanced ATP-induced current, while its longer application partially suppressed this current. In most submucous neurons, acetylcholine (ACh) likewise activated an inward cationic current. The amplitude of ACh-induced current was lower if ACh was applied during a long-lasting application of ATP than if ACh only was applied. Hexamethonium (50 µM), d-tubocurarine (20-40 µM), and trimethaphan (30 µM) completely and reversibly blocked ACh-induced currents, regardless of the presence of ATP, and did not affect ATP-induced currents. The results suggest that ATP-induced currents in submucous neurons are due to activation of a unique type of P2 purinoreceptors, which function in connection with nicotinic ACh receptors. Plenum Publishing Corporation, 1997 Glushakov A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Melishchuk A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Skok V. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/2-3(1996-03-01), 77-85 0090-2977 28:2-3<77 1996 28 11062 https://doi.org/10.1007/BF02262767 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02262767 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Glushakov A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Melishchuk A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 700 1- Skok V. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 28/2-3(1996-03-01), 77-85 0090-2977 28:2-3<77 1996 28 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer