caa a22 4500 477075002 CHVBK 20180405111437.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02827590 doi (NATIONALLICENCE)springer-10.1007/BF02827590 Studies on reduced extent method for inhibited thermokinetics of enzyme-catalyzed reaction [Elektronische Daten] Inhibition of the laccase-catalyzed oxidation of o -dihydroxybenzene by m -dihydroxybenzene [Xiong Ya, Wu Dingquan, Qu Songsheng, Du Yumin] The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distinguishing inhibition type was given and the methods for calculating kinetic parameters,K M,K i andv m were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type,K M=6.224×10−3 mol·L−1,K i=2.363×10−2 mol·L−1. Springer, 1996 thermokinetics nationallicence enzyme-catalyzed reaction nationallicence inhibition nationallicence reduced extent method nationallicence laccase nationallicence Ya Xiong Department of Chemistry, Wuhan University, 430072, Wuhan, China aut Dingquan Wu Department of Chemistry, Wuhan University, 430072, Wuhan, China aut Songsheng Qu Department of Chemistry, Wuhan University, 430072, Wuhan, China aut Yumin Du Department of Environmental Science, Wuhan University, 430072, Wuhan, China aut Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 98-100 1007-1202 1:1<98 1996 1 11859 https://doi.org/10.1007/BF02827590 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02827590 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ya Xiong Department of Chemistry, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Dingquan Wu Department of Chemistry, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Songsheng Qu Department of Chemistry, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yumin Du Department of Environmental Science, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 98-100 1007-1202 1:1<98 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer