caa a22 4500 477075045 CHVBK 20180405111437.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02827594 doi (NATIONALLICENCE)springer-10.1007/BF02827594 Preparation of exine-detached pollen in Nicotiana tabacum [Elektronische Daten] [Xia Huijun, Zhou Chang, Yang Hongyuan] A method of preparing exine-detached pollen inNicotiana tabacum was established. Anthers containing early-middle binucleate pollen were cold-pretreated at 4-6°C for 7-14 days, and were suspended in 0.3 mol/L sucrose solution for 2 days. During this process, the exine of most pollen grains dehisced. Then they were transferred into an enzyme solution containing 1% cellulase, 1% pectinase, 0.1% pectolyase, 1 mol/L mannitol, 0.3 mol/L sorbitol, 0.5% potassium dextran sulphate and K3 medium macro elements. After 15-20 min enzymatic maceration, the exine was detached resulting in the release of exine-detached pollen. Factors affecting preparation of exine-detached pollen were investigated, including cold-pretreatment, osmoticum concentration and enzymes used. Springer, 1996 exine-detached pollen nationallicence isolation nationallicence Nicotiana tabacum nationallicence Huijun Xia College of Life Sciences, Wuhan University, 430072, Wuhan aut Chang Zhou College of Life Sciences, Wuhan University, 430072, Wuhan aut Hongyuan Yang College of Life Sciences, Wuhan University, 430072, Wuhan aut Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 116-118 1007-1202 1:1<116 1996 1 11859 https://doi.org/10.1007/BF02827594 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02827594 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huijun Xia College of Life Sciences, Wuhan University, 430072, Wuhan aut NATIONALLICENCE 700 1- Chang Zhou College of Life Sciences, Wuhan University, 430072, Wuhan aut NATIONALLICENCE 700 1- Hongyuan Yang College of Life Sciences, Wuhan University, 430072, Wuhan aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 116-118 1007-1202 1:1<116 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer