caa a22 4500 477075096 CHVBK 20180405111437.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02827595 doi (NATIONALLICENCE)springer-10.1007/BF02827595 Studies in in situ PCR detected by the BrdU antibody technique [Elektronische Daten] [Zhang Xiyuan, Jiang Haibo, Ma Qi, Yang Jianqi, Li Lijia, Liu Ting, Yan Ju] Using the BrdU antibody technique followed by an immuno-chemical staining (BAT), the amplification of DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products orin situ hybridization with PCR products on slides, the amplified target DNA fragments of specimen were visualized by BAT under the microscope. The availability of BAT and differences in the sensitivity and efficiency between BAT and dig-11-dUTP labeling in cellin situ PCR were discussed. Springer, 1996 BrdU antibody technique nationallicence cell in situ PCR nationallicence in situ hybridization nationallicence human Y chromosome nationallicence polymerase chain reaction (PCR) nationallicence Xiyuan Zhang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Haibo Jiang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Qi Ma College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Jianqi Yang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Lijia Li College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Ting Liu College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Ju Yan Genetics Lab of Tongji Medical University, 430030, Wuhan aut Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 119-124 1007-1202 1:1<119 1996 1 11859 https://doi.org/10.1007/BF02827595 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02827595 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiyuan Zhang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Haibo Jiang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Qi Ma College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Jianqi Yang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Lijia Li College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ting Liu College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ju Yan Genetics Lab of Tongji Medical University, 430030, Wuhan aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/1(1996-03-01), 119-124 1007-1202 1:1<119 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer