caa a22 4500 477075347 CHVBK 20180405111437.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02901238 doi (NATIONALLICENCE)springer-10.1007/BF02901238 Rice protoplasts isolated directly from mature-embryo-derived calli [Elektronische Daten] [Jin Deming, Maria-Jesus Cornejo] Mature-embryo-derived calli of japonica rice (Oryza sativa L) Taipei 309 were used for replicated protoplast isolation experiments. Six out of nine callus lines produced protoplasts with satisfactory yield of 5.20×106-8.96×106 protoplasts/g FW (fresh weight). The remaining three callus lines initiated from seeds of cryopreserved-callus-derived plants had rooty calli, resulting in low yield of protoplasts and a large number of isolated banana-shape intact cells. Viability of protoplasts ranged 87.46%-94.15%. The average size of protoplasts was 207.49-379.04 μm2 in different callus lines. Comparitive experiments were also carried out using both calli and suspension culture cells for protoplast isolation. The results demonstrated that protoplast isolation of calli was a substantially simplified and reliable method for preparing rice protoplasts. Springer, 1996 rice ( Oryza sativa L) nationallicence protoplast isolation nationallicence fluorecence dye nationallicence confocal laser scanning microscopy nationallicence Deming Jin Department of Agronomy, Huazhong Agricultural University, 430070, Wuhan, China aut Cornejo Maria-Jesus Department of Plant Biology, University of Valencia, 46100, Burjassot, Spain aut Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 251-258 1007-1202 1:2<251 1996 1 11859 https://doi.org/10.1007/BF02901238 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02901238 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deming Jin Department of Agronomy, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Cornejo Maria-Jesus Department of Plant Biology, University of Valencia, 46100, Burjassot, Spain aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 251-258 1007-1202 1:2<251 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer