caa a22 4500 477075398 CHVBK 20180405111438.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02901241 doi (NATIONALLICENCE)springer-10.1007/BF02901241 Studies on introduction of arrowhead proteinase inhibitor gene into N. tobacco protoplasts [Elektronische Daten] [Wang Xin, Xie Weijun, Ma Xiaojun, Gong Zhenzhen] The Arrowhead Proteinase Inhibitor (API) gene was introduced into the protoplasts of mesophyll cells ofN. tobacco by PEG-mediated. The transformed protoplasts underging differentiation of callus and regeneration of plantlet have been growing into transgenic plants. Restriction endonuclease analysis of products amplificated by PCR indicates the existence of the API gene in the transformed plantlet. The extract of the leaves from the transformed plants shows trypsin inhibitory activity, which indicates the expression on the introduced API gene and the transformed plants can accumulate the inhibitor. However, the variation of the inhibitory activity of the transformed plants reveals the importance of the integration site of the API gene in the genome. Springer, 1996 polyethyleneglycol mediated gene transfer nationallicence arrowhead Proteinase inhibitor gene nationallicence transgenic tobacco nationallicence Xin Wang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Weijun Xie Shanghai Institute of Biochemistry Academia Sinica, 200031, Shanghai, China aut Xiaojun Ma College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Zhenzhen Gong Shanghai Institute of Biochemistry Academia Sinica, 200031, Shanghai, China aut Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 267-271 1007-1202 1:2<267 1996 1 11859 https://doi.org/10.1007/BF02901241 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02901241 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xin Wang College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Weijun Xie Shanghai Institute of Biochemistry Academia Sinica, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Xiaojun Ma College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhenzhen Gong Shanghai Institute of Biochemistry Academia Sinica, 200031, Shanghai, China aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 267-271 1007-1202 1:2<267 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer