caa a22 4500 477075495 CHVBK 20180405111438.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02901243 doi (NATIONALLICENCE)springer-10.1007/BF02901243 Expression of E1 gene of a hepatitis C virus in E. coli and protein purification [Elektronische Daten] [Ye Linbai, Gao Jinrong, Meng Xiaolin, Xu Jinping, Zhu Ying, Hu Min, Min Lei, Ye Chanying, Wu Zhenhue] The cDNA containing full encoding region of E1 antigen of HCV was cloned into an expression plasmid pRSETHisB. The recombinant plasmid pRSETE1 was introduced into the BL21 (DE3) strain ofE. coli. The engineering bacteria harbouring the pRSETE1 was cultivated in 2YT medium at 37°C. When the Expression of E1 protein was induced by 1 mmol IPTG, the bacteria was killed and the number of living cell was droped down from 107 to 103 cell/mL one hour post induction. Suggest that E1 protein is poisoned toE. coli. However, the 26kD polypeptide of E1 fussion protein still synthesized in appropriate condition. The expression level was about 10% of total protein 4 h after inducing. The E1 protin was purified by Ni2+-NTA-Agarose column chromatography to homogeneous. The purified E1 protein was sensitive and specific in reaction with anti-HCV antibody in sera. Springer, 1996 HCV E1 antigen nationallicence expression nationallicence purification nationallicence Linbai Ye National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Jinrong Gao National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Xiaolin Meng National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Jinping Xu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Ying Zhu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Min Hu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Lei Min National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Chanying Ye National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Zhenhue Wu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 279-282 1007-1202 1:2<279 1996 1 11859 https://doi.org/10.1007/BF02901243 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02901243 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linbai Ye National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Jinrong Gao National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Xiaolin Meng National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Jinping Xu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ying Zhu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Min Hu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Lei Min National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Chanying Ye National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhenhue Wu National Lab. of Virology and Biocontrol, Institute of Virology and Department of Virology and Molecular Biology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 279-282 1007-1202 1:2<279 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer