caa a22 4500 477075509 CHVBK 20180405111438.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02901242 doi (NATIONALLICENCE)springer-10.1007/BF02901242 Nucleotide sequence of polyhedrin gene of LsNPV and analysis of baculovirus polyhedron proteins [Elektronische Daten] [Wang Jiawang, Qi Yipeng, Deng Yanhui, Mallam Joshua] The intact 741 bp polyhedrin gene of LsNPV was sequenced by Silver, Sequencing System, and shares 90.6% and 97.0% nucleotide identity, 97.2% and 97.6% amino acid identity with PfNPV and MdNPV polh genes respectively. The 14 bp conservative sequence with the core element GTAAG, is located in the 5′untranslated region of the gene. The polh gene was predicted to encodes a 246 amino acid residures with molecular weight of 29.0 kd, in which the number of acidic amino acids and alkaline amino acids was roughly equal resulting in almost no charges in polyhedrin protein molecule and hence occlusion body. It gives a valuable implication that ionic bonds as well as hydrophobic bonds and hydrogen bond may play an important role in the crystallization of polyhedrin, by comparing amino acid variation of twenty—one polyhedrin. The comparsion of promoter regions of polyhedrin gene and class II gene shown that they are very similar, but also have differences in GC content. This could explain that both categories of gene are highly expressed, and polyhedrin genes are expressed more higher than class II gene. Springer, 1996 LsNPV nationallicence baculovirus nationallicence polyhedrin gene nationallicence sequence nationallicence homology nationallicence Jiawang Wang Institute of Virology, Wuhan University, 430072, Wuhan, China aut Yipeng Qi Institute of Virology, Wuhan University, 430072, Wuhan, China aut Yanhui Deng Institute of Virology, Wuhan University, 430072, Wuhan, China aut Joshua Mallam Institute of Virology, Wuhan University, 430072, Wuhan, China aut Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 272-278 1007-1202 1:2<272 1996 1 11859 https://doi.org/10.1007/BF02901242 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02901242 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiawang Wang Institute of Virology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yipeng Qi Institute of Virology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yanhui Deng Institute of Virology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Joshua Mallam Institute of Virology, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Wuhan University Journal of Natural Sciences Wuhan University 1/2(1996-06-01), 272-278 1007-1202 1:2<272 1996 1 11859 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer