caa a22 4500 477076076 CHVBK 20180405111438.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02514970 doi (NATIONALLICENCE)springer-10.1007/BF02514970 Nutritional contribution to females of14C-labeled male secretions transferred during mating in Menida scotti (Heteroptera: Pentatomidae) [Elektronische Daten] [Yozo Koshiyama, Hisaaki Tsumuki, Kenji Fujisaki, Fusao Nakasuji] Menida scotti (Puton) males have been shown to transfer secretions from their bulbus ejaculatorius and reservoir of ectodermal accessory gland to females by mating during hibernation. In the present study, the major components of the secretions were found to be proteins and lipids. To specify the female organ incorporating the male secretions, a radiotracer experiment in which the male secretions were labeled by [14C]valine was conducted in nine tissues of females collected in the fall and spring of the hibernation period. Relatively high radioactivities were detected in the haemolymph and the residual carcass (head, legs, air-sacs, exoskeleton, etc.) in the fall females, and in CO2 gas evolved and carcasses in the spring females. The radioactivities in the fat body were significantly higher in the fall mating females than in the spring mating females, and vice versa in the ovary. The radioactivities in six fractions (lipids, proteins, glycogen, sugars, free amino acids and the residues) were also assayed in the five organs of females that had a relatively high radioactivity. The highest radioactivity was detected in the protein fraction of the haemolymph in fall and spring females. There were significant differences in the radioactivities incorporated into the lipid fractions of the carcass between fall and spring females. Society of Population Ecology, 1996 accessory gland secretion nationallicence diapause nationallicence hibernation nationallicence male-derived nutrients nationallicence radiotracer experiment nationallicence reproduction nationallicence Koshiyama Yozo Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut Tsumuki Hisaaki Research Institute for Bioresources, Okayama University, 710, Kurashiki, Japan aut Fujisaki Kenji Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut Nakasuji Fusao Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut Researches on Population Ecology Springer-Verlag 38/1(1996-06-01), 51-56 0034-5466 38:1<51 1996 38 10144 https://doi.org/10.1007/BF02514970 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02514970 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koshiyama Yozo Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Tsumuki Hisaaki Research Institute for Bioresources, Okayama University, 710, Kurashiki, Japan aut NATIONALLICENCE 700 1- Fujisaki Kenji Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Nakasuji Fusao Faculty of Agriculture, Okayama University, 700, Okayama, Japan aut NATIONALLICENCE 773 0- Researches on Population Ecology Springer-Verlag 38/1(1996-06-01), 51-56 0034-5466 38:1<51 1996 38 10144 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer