caa a22 4500 477080995 CHVBK 20180405111449.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02722993 doi (NATIONALLICENCE)springer-10.1007/BF02722993 Stimulation of endothelial cell migration in culture by ladsin, a laminin-5-like cell adhesion protein [Elektronische Daten] [Yamato Kikkawa, Kotaro Akaogi, Hiroto Mizushima, Naoki Yamanaka, Makoto Umeda, Kaoru Miyazaki] Summary: Ladsin is a laminin-like cell-adhesive scatter factor with potent cell motility-stimulating ability and was purified from serum-free conditioned medium of a malignant human gastric adenocarcinoma cell line STKM-1. To test its possible role in tumor angiogenesis, we investigated its effect on primary culture of endothelial cells (human umbilical vein endothelial cells) and endothelial cell line ECV304 in this study. Cell adhesion and motility effects of ladsin were observed in both types of endothelial cells. In cell-attachment assay, ladsin interacted with integrin α3β1 that was expressed on the endothelial cell surface. In Boyden chambers, ladsin stimulated both directed and random migration of ECV304 cells. Ladsin induced repair of artificial wounds generated in ECV304 cell monolayers by stimulating cell migration. Ladsin did not affect the growth rate of ECV304 cells at a low cell density but significantly increased the saturation cell density. These results suggest that ladsin may be involved in the adhesion and migration of endothelial cells under some physiological and pathological conditions. Society for In Vitro Biology, 1996 endothelial cell nationallicence cell migration nationallicence cell adhesion nationallicence laminin nationallicence tumor angiogenesis nationallicence Kikkawa Yamato Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut Akaogi Kotaro Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut Mizushima Hiroto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut Yamanaka Naoki Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut Umeda Makoto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut Miyazaki Kaoru Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/1(1996-01-01), 46-52 1071-2690 32:1<46 1996 32 11626 https://doi.org/10.1007/BF02722993 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722993 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kikkawa Yamato Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 700 1- Akaogi Kotaro Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 700 1- Mizushima Hiroto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 700 1- Yamanaka Naoki Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 700 1- Umeda Makoto Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 700 1- Miyazaki Kaoru Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, 244, Yokohama, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/1(1996-01-01), 46-52 1071-2690 32:1<46 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer