caa a22 4500 477081029 CHVBK 20180405111449.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02722990 doi (NATIONALLICENCE)springer-10.1007/BF02722990 Differential release of prostaglandins by organ cultures of human fetal trachea and lung [Elektronische Daten] [Robert Hume, Jeanne Bell, David Cossar, Merete Giles, Anne Hallas, Rodney Kelly] Summary: Human fetal lung at 16-19 weeks gestation has a partially differentiated epithelium, and in organ culture, distal airsacs dilate and the epithelium autodifferentiates to type I and II pneumatocytes, processes regulated by endogenous prostaglandin PGE2. Human fetal trachea, at the same gestation, has a terminally differentiated mucociliary epithelium but after 4-6 d in organ culture, develops squamous metaplasia. Tracheal cultures restricted to 3 d have normal phase-contrast and light microscopy appearances and immunohistochemical reactivities (epithelium: cytokeratin 7,8,18; glutathione S-transferase pi-isozyme; epithelial membrane antigen and mesenchyme; desmin; vimentin). In human fetal trachea organ cultures, the predominant prostaglandins released are 6-keto-PGF1α, PGF2α, and PGE2, a pattern similar to that previously described for human adult trachea and lung. In fetal lung cultures, 13,14-dihydro-15-keto-PGF2α is the major prostaglandin released with lesser amounts of 13,14-dihydro-15-keto-PFG2α, PGF2α, PGE2, and 6-keto-PGF1α. Human fetal lungin vitro has the competence to self-differentiate, as early as 12 weeks gestation and presence of high levels in fetal lung of the inactive metabolite 13,14-dihydro-15-keto-PGE2 relative to PGE2 suggests that active prostaglandin catabolism may be one of the mechanisms to retard this stage of maturationin vivo by limiting PGE2 availability. Surprisingly, the profile of prostaglandins released from fetal lung organ culture does not change to that of a mature lung with terminal differentiation of the epithelium, and this may indicate differences in the expression of key prostaglandin-metabolizing enzymes in developing human fetal lung in culture and within utero ontogeny. Society for In Vitro Biology, 1996 development nationallicence immunohistochemistry nationallicence differentiation nationallicence Hume Robert Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut Bell Jeanne Department of Pathology, University of Edinburgh, Edinburgh, Scotland, UK aut Cossar David Department of Pathology, University of Edinburgh, Edinburgh, Scotland, UK aut Giles Merete Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut Hallas Anne Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut Kelly Rodney MRC Centre for Reproductive Biology, Edinburgh, Scotland, UK aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/1(1996-01-01), 24-29 1071-2690 32:1<24 1996 32 11626 https://doi.org/10.1007/BF02722990 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722990 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hume Robert Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut NATIONALLICENCE 700 1- Bell Jeanne Department of Pathology, University of Edinburgh, Edinburgh, Scotland, UK aut NATIONALLICENCE 700 1- Cossar David Department of Pathology, University of Edinburgh, Edinburgh, Scotland, UK aut NATIONALLICENCE 700 1- Giles Merete Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut NATIONALLICENCE 700 1- Hallas Anne Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, DD1 9SY, Dundee, Scotland, UK aut NATIONALLICENCE 700 1- Kelly Rodney MRC Centre for Reproductive Biology, Edinburgh, Scotland, UK aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/1(1996-01-01), 24-29 1071-2690 32:1<24 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer