caa a22 4500 477081045 CHVBK 20180405111449.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02722963 doi (NATIONALLICENCE)springer-10.1007/BF02722963 Monoclonal antibodies against human chondrocytes [Elektronische Daten] [J. Bujia, M. Sittinger, H. Sudhoff, A. Fisseler-Eckhoff, G. Burmester, E. Kastenbauer, C. Hammer] Summary: Cell-specific antigens are mainly found in cells or membrane surfaces rather than in the surrounding matrix. However, until now it was not possible to produce antibodies specific for cellular structures of chondrocytes. In 1989, Lance (Immunol. Lett. 21:63-73; 1989) first established specific monoclonal antibodies for human articular chondrocytes tested only by immunofluorescence. Studies describing the specificity of these five antibodies (HUMC 1-5) and their relevance for immunohistological analysis of cartilage tissue were not available until now. Therefore, the aim of the following study was to investigate the distribution of HUMC 1, 2, 3, 4, and 5 in mesenchymal cellsin vivo andin vitro immunohistochemically. Further investigations concentrate on the localization of chondrocyte specific antigens using immunoelectron microscopy. Immunohistological studies showed positive immunostainings with all five antibodies in human chondrocytesin vivo andin vitro. A cross-reaction with human fibroblasts and osteoblasts for the antibodies HUMC 2 and HUMC 5 was observed. furthermore, a parallel loss of immunoreactivity for HUMC 1, HUMC 3, and HUMC 4 was observed in cultured chondrocytes indicating that the specific antigens vanish during differentiation observedin vitro. Subsequent immunoblot analysis employing collagens as antigens did not show any reactivity. Using immunoelectron microscopy, gold particle labeling was observed in intracytoplasmatic vesicles of isolated chondrocytes. Our results indicate that HUMC 1, HUMC 3, and HUMC 4 are specific for cartilage cells and might be suitable for immunohistological analysis of different cartilage tissues and pathologically altered chondrocytes. Society for In Vitro Biology, 1996 cartilage differentiation nationallicence monoclonal antibodies nationallicence immunohistochemistry nationallicence western blotting nationallicence immunoelectron microscopy nationallicence Bujia J. Department of Otolaryngology, Ludwig-Maximilians-University of Munich, Germany aut Sittinger M. Department of Medicine III, Charité, Humboldt University of Berlin, Germany aut Sudhoff H. Department of Otorhinolaryngology, St. Elisabeth Hospital, Germany aut Fisseler-Eckhoff A. Institute of Pathology, Berufsgenossenschaftliche Kliniken "Bergmannsheil” University of Bochum, Germany aut Burmester G. Department of Medicine III, Charité, Humboldt University of Berlin, Germany aut Kastenbauer E. Department of Otolaryngology, Ludwig-Maximilians-University of Munich, Germany aut Hammer C. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Germany aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 366-371 1071-2690 32:6<366 1996 32 11626 https://doi.org/10.1007/BF02722963 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722963 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujia J. Department of Otolaryngology, Ludwig-Maximilians-University of Munich, Germany aut NATIONALLICENCE 700 1- Sittinger M. Department of Medicine III, Charité, Humboldt University of Berlin, Germany aut NATIONALLICENCE 700 1- Sudhoff H. Department of Otorhinolaryngology, St. Elisabeth Hospital, Germany aut NATIONALLICENCE 700 1- Fisseler-Eckhoff A. Institute of Pathology, Berufsgenossenschaftliche Kliniken "Bergmannsheil” University of Bochum, Germany aut NATIONALLICENCE 700 1- Burmester G. Department of Medicine III, Charité, Humboldt University of Berlin, Germany aut NATIONALLICENCE 700 1- Kastenbauer E. Department of Otolaryngology, Ludwig-Maximilians-University of Munich, Germany aut NATIONALLICENCE 700 1- Hammer C. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Germany aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 366-371 1071-2690 32:6<366 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer