caa a22 4500 477081061 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02722959 doi (NATIONALLICENCE)springer-10.1007/BF02722959 Novel Madin Darby Canine Kidney cell clones exhibit unique phenotypes in response to morphogens [Elektronische Daten] [Stephanie Orellana, Christopher Neff, William Sweeney, Ellis Avner] Summary: Novel Madin Darby Canine Kidney cell clones were isolated. These cell clones exhibit differential responsiveness to inducers of tubule or cyst formation in collagen gel culture: hepatocyte growth factor or inducers of intracellular cAMP formation, respectively. In gel culture, clone OR93.22.D6 forms cysts and responds with morphological transformation to both hepatocyte growth factor and prostaglandin E1, and is most typical of a previously described cell type except for its higher transepithelial electrical resistance. OR55.25.II20 forms tubules in culture, is unresponsive to hepatocyte growth factor, and forms prostaglandin-induced spherical cysts. OR55.28.V2 forms dense cell spheres under control conditions, is induced to form tubules by hepatocyte growth factor, and is unresponsive to prostaglandin. OR55.29 forms only cysts, and is the only clone to form domes in monolayer culture. Tubule formation induced by hepatocyte growth factor, in all clones except OR55.25.II20, is blocked by a neutralizing antibody. In defined medium, without hepatocyte growth factor or prostaglandin, OR55.25.II20 forms spontaneous tubules. This finding indicates that a tubulogenic serum factor is not responsible for the observed phenotype. Increasing prostaglandin concentrations lead to inhibition of tubule formation and increased cyst formation. This observation suggests that induction of intracellular cAMP formation negatively regulates tubule formation in these cells, and implies that cystogenesis may represent a "default pathway” for impaired tubulogenesis. These observations demonstrate that some facets of renal tubulogenesis may be independent of hepatocyte growth factor, and that care must be exercised when comparing biological studies utilizing different clones. Society for In Vitro Biology, 1996 nephrogenesis nationallicence growth factors nationallicence morphogenesis nationallicence Orellana Stephanie Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut Neff Christopher Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut Sweeney William Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut Avner Ellis Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 329-339 1071-2690 32:6<329 1996 32 11626 https://doi.org/10.1007/BF02722959 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722959 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orellana Stephanie Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut NATIONALLICENCE 700 1- Neff Christopher Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut NATIONALLICENCE 700 1- Sweeney William Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut NATIONALLICENCE 700 1- Avner Ellis Department of Pediatrics, University of Washington and Division of Nephrology, Children's Hospital and Medical Center, Seattle, Washington aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 329-339 1071-2690 32:6<329 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer