caa a22 4500 477081096 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02722962 doi (NATIONALLICENCE)springer-10.1007/BF02722962 Mycoplasma orale infection affects K+ and Cl− currents in the HSG salivary gland cell line [Elektronische Daten] [Kenneth Izutsu, Sahba Fatherazi, Carol Belton, Dolphine Oda, Frank Cartwright, George Kenny] Summary: The relations between K+ channel and Cl− channel currents and mycoplasma infection status were studied longitudinally in HSG cells, a human submandibular gland cell line. The K+ channel currents were disrupted by the occurrence of mycoplasma infection: muscarinic activation of K+ channels and K+ channel expression as estimated by ionomycin- or hypotonically induced K+ current responses were all decreased. Similar decreases in ionomycin- and hypotonically induced responses were observed for Cl− channels, but only the latter decrease was statistically significant. Also, Cl− currents could be elicited more frequently than K+ currents (63% of cases versus 0%) in infected cells when tested by exposure to hypotonic media, indicating that mycoplasma infection affects K+ channels relatively more than Cl− channels. These changes occurred in the originally infected cells, were ameliorated when the infection was cleared with sparfloxacin, and recurred when the cells were reinfected. Such changes would be expected to result in hyposecretion of salivary fluid if they occurredin vivo. Society for In Vitro Biology, 1996 potassium channels nationallicence chloride channels nationallicence submandibular gland nationallicence infection nationallicence Izutsu Kenneth Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut Fatherazi Sahba Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut Belton Carol Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut Oda Dolphine Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut Cartwright Frank Department of Pathobiology, School of Medicine, University of Washington, 98195, Seattle, Washington aut Kenny George Department of Pathobiology, School of Medicine, University of Washington, 98195, Seattle, Washington aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 361-365 1071-2690 32:6<361 1996 32 11626 https://doi.org/10.1007/BF02722962 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722962 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Izutsu Kenneth Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 700 1- Fatherazi Sahba Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 700 1- Belton Carol Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 700 1- Oda Dolphine Department of Oral Biology, School of Dentistry, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 700 1- Cartwright Frank Department of Pathobiology, School of Medicine, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 700 1- Kenny George Department of Pathobiology, School of Medicine, University of Washington, 98195, Seattle, Washington aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 361-365 1071-2690 32:6<361 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer