caa a22 4500 47708110X CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02722958 doi (NATIONALLICENCE)springer-10.1007/BF02722958 Release of chromaffin granular content from staphylococcal enterotoxin B (SEB)-treated and-untreated PC12 cells [Elektronische Daten] [Tatsuo Hase, Marti Jett, Edward Asafo-Adjei, Michael Topper] Summary: The release of chromaffin granular content from staphylococcal enterotoxin B (SEB)-treated and-untreated PC12 cells was studied by electron microscopy. The treatment of the cells with SEB at the concentration of 20 μg/ml caused marked increase of the chromaffin granules that either bound to the plasma membrane by the characteristic rods, measuring 15 to 20 nm in length and showing a tubular structure, or budded off at the free cell surface, surrounded by a layer of rod-containing cytoplasm and enclosed by the plasma membrane. The binding between the granular and plasma membranes by the rods did not lead to membrane fusion and exocytosis of the granular content. Many of the bound granules showed vesiculation with loss of the electron-dense core material; at the same time, some of the binding rods contained intraluminal electron-dense material similar to the granular core material. These findings suggested that the electron-dense material (i.e., norepinephrine) of the bound granules was released extracellularly through channels within the rods. Although the granules were bound to the plasma membrane with equal frequency at the free and contiguous cell surfaces, the granular budding occurred only at the free cell surface, indicating that it occurred incidentally to some granules bound at the free cell surfaces. On the basis of the morphological observations, it is postulated that the electron-dense material of the bound granule is selectively released extracellularly through the rods, leaving the vesiculated granules behind in the cytoplasm. The same mode of release of the granular content was observed, though less frequently, in the untreated control cells. No morphological evidence that indicated that the granular content was released extracellularly by exocytosis was found in the treated and control cells. The present observations indicated that the SEB treatment of PC12 cells stimulated the binding of chromaffin granules to the plasma membrane by the rods and the budding of the bound granules at the free cell surface. Society for In Vitro Biology, 1996 Chromaffin granule nationallicence Electron microscopy nationallicence PC12 cell nationallicence Staphylococcal enterotoxin B(SEB) nationallicence Hase Tatsuo Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut Jett Marti Department of Molecular Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut Asafo-Adjei Edward Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut Topper Michael Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 322-328 1071-2690 32:6<322 1996 32 11626 https://doi.org/10.1007/BF02722958 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722958 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hase Tatsuo Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut NATIONALLICENCE 700 1- Jett Marti Department of Molecular Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut NATIONALLICENCE 700 1- Asafo-Adjei Edward Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut NATIONALLICENCE 700 1- Topper Michael Department of Ultrastructural Pathology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 322-328 1071-2690 32:6<322 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer