caa a22 4500 477081126 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02722964 doi (NATIONALLICENCE)springer-10.1007/BF02722964 Efficient production of IGG human monoclonal antibodies by lymphocytes stimulated by lipopolysaccharide, pokeweed mitogen, and interleukin 4 [Elektronische Daten] [Kaoru Yoshinari, Kenji Arai, Hideki Kimura, Kunio Matsumoto, Yutaka Yamaguchi] Summary: Extensive screening of the mitogens lipopolysaccharide (LPS), pokeweed mitogen (PWM), andStaphylococcus aureus Cowan I (SAC I), alone and in combination and with and without interleukin (IL) was performed forin vitro activation of regional lymph node lymphocytes from lung cancer patients for the production of human IgG, IgM, and IgA. As assessed by electrofusion of the lymphocytes following their exposure to these agents with mouse myeloma cells and incubation of the fused hybridoma, a remarkable stimulatory effect was shown by LPS and particularly by LPS plus IL-4, which was substantially greater than that of either SAC I or PWM with or without various IL. Optimization studies indicated that the addition of PWM to LPS and IL-4 in the culture medium further stimulated the human antibody (Ab) production, and that the optimal formulation for stimulations of human IgG production was a culture medium containing 20 μg/ml of LPS, 1/500 of PWM, and 100 u/ml of IL-4. Society for In Vitro Biology, 1996 monoclonal antibody, human nationallicence lipopolysacharide nationallicence pokeweed mitogen nationallicence interleukin 4 nationallicence Yoshinari Kaoru Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut Arai Kenji Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut Kimura Hideki Division of Chest Diseases, Chiba Cancer Center, 280, Chiba, Japan aut Matsumoto Kunio Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut Yamaguchi Yutaka Department of Surgery, Institute of Pulmonary Cancer Research, School of Medicine, Chiba University, 280, Chiba, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 372-377 1071-2690 32:6<372 1996 32 11626 https://doi.org/10.1007/BF02722964 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722964 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshinari Kaoru Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut NATIONALLICENCE 700 1- Arai Kenji Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut NATIONALLICENCE 700 1- Kimura Hideki Division of Chest Diseases, Chiba Cancer Center, 280, Chiba, Japan aut NATIONALLICENCE 700 1- Matsumoto Kunio Diagnostics R&D Dept., Asahi Chemical Industry Co. Ltd., 632-1, Mifuku, Ohito-cho, 410-23, Shizuoka, Japan aut NATIONALLICENCE 700 1- Yamaguchi Yutaka Department of Surgery, Institute of Pulmonary Cancer Research, School of Medicine, Chiba University, 280, Chiba, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/6(1996-06-01), 372-377 1071-2690 32:6<372 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer