caa a22 4500 477081169 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF02722982 doi (NATIONALLICENCE)springer-10.1007/BF02722982 Establishment and characterization of a novel human myoepithelial cell line and matrix-producing xenograft from a parotid basal cell adenocarcinoma [Elektronische Daten] [Mark Sternlicht, Suzi Safarians, Thomas Calcaterra, Sanford Barsky] Summary: Myoepithelial cells exert important paracrine effects on epithelial morphogenesis and mitogenesis through direct cell-cell interactions and through synthesis of a basement membrane extracellular matrix. To study these effects further, this study established the first immortalized human myoepithelial cell line, HMS-1, and transplantable xenograft, HMS-X, from the rare parotid basal cell adenocarcinoma. The cell line exhibited a fully differentiated myoepithelial phenotype and the xenograft exhibited the rare property of accumulating an abundant extracellular matrix composed of both basement membrane and nonbasement membrane components with the latter predominating. With HMS-1 as a feeder layer, dramatic and specific induction of epithelial morphogenesis (sheroid formation) occurred with selected normal epithelial and primary carcinoma target cells. HMS-1 and HMS-X provide distinct advantages over the conventional murine matrices in existence. They will be invaluable in future studies of human tumor-myoepithelial and matrix interactions important for tumor cell growth, invasion, and metastasis. Society for In Vitro Biology, 1996 salivary gland tumors nationallicence myoepithelial cells nationallicence extracellular matrix nationallicence morphogenesis nationallicence Sternlicht Mark Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut Safarians Suzi Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut Calcaterra Thomas Department of Surgery, Head and Neck Division, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut Barsky Sanford Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 550-563 1071-2690 32:9<550 1996 32 11626 https://doi.org/10.1007/BF02722982 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722982 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sternlicht Mark Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut NATIONALLICENCE 700 1- Safarians Suzi Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut NATIONALLICENCE 700 1- Calcaterra Thomas Department of Surgery, Head and Neck Division, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut NATIONALLICENCE 700 1- Barsky Sanford Department of Pathology, UCLA School of Medicine, 10833 Le Conte Avenue, 90024-1732, Los Angeles, California aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 550-563 1071-2690 32:9<550 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer