caa a22 4500 477081193 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF02722984 doi (NATIONALLICENCE)springer-10.1007/BF02722984 The effect of angiotensin II on myosin heavy chain expression in cultured myocardial cells [Elektronische Daten] [Noa Shalitin, Menahem Friedman, Hadassa Schlesinger, Yael Barhum, Maurice Levy, Wolfgang Schaper, Gania Kessler-Icekson] Summary: Angiotensin II (AII), the principal mediator of the renin-angiotensin system, is an important regulator of vascular and cardiac homeostasis. AII has also been shown to be a regulator of cardiac hypertrophy and of the corresponding changes in amount and composition of certain tissue proteins. We examined the trophic effects of AII on cultured myocytes derived from neonatal rat ventricles and followed, by Northern blot analysis and polyacrylamide gel electrophoresis, the expression of α- and β-myosin heavy chain iso-mRNAs and isoproteins. Our findings show that a single administration of AII is sufficient to induce a trophic response in cultured beating myocytes and to enhance the expression of β-myosin heavy chain iso-mRNA and isoprotein, having no effect on α-myosin heavy chain. Induction of α-myosin heavy chain expression by thyroid hormone before AII was administered showed that AII could not potentiate a shift from α- to β-myosin heavy chain predominance. We suggest that the potency of AII to regulate the expression of myosin heavy chain isogenes is restricted to the β isoform and is overridden by thyroid hormone. Society for In Vitro Biology, 1996 neonatal heart myocytes nationallicence angiotensin II nationallicence cellular hypertrophy nationallicence myosin heavy chains nationallicence Shalitin Noa The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut Friedman Menahem Department of Surgery, Sackler School of Medicine, Tel-Aviv University, Israel aut Schlesinger Hadassa The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut Barhum Yael The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut Levy Maurice Department of Surgery, Sackler School of Medicine, Tel-Aviv University, Israel aut Schaper Wolfgang Department of Experimental Cardiology, MPI, Bad-Nauheim, Federal Republic of Germany aut Kessler-Icekson Gania The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 573-578 1071-2690 32:9<573 1996 32 11626 https://doi.org/10.1007/BF02722984 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722984 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shalitin Noa The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut NATIONALLICENCE 700 1- Friedman Menahem Department of Surgery, Sackler School of Medicine, Tel-Aviv University, Israel aut NATIONALLICENCE 700 1- Schlesinger Hadassa The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut NATIONALLICENCE 700 1- Barhum Yael The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut NATIONALLICENCE 700 1- Levy Maurice Department of Surgery, Sackler School of Medicine, Tel-Aviv University, Israel aut NATIONALLICENCE 700 1- Schaper Wolfgang Department of Experimental Cardiology, MPI, Bad-Nauheim, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kessler-Icekson Gania The Basil and Gerald Felsenstein Medical Research Center, Beilinson Medical Campus, 49100, Petah-Tikva, Israel aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 573-578 1071-2690 32:9<573 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer